Journal ArticleDOI
Quantitative analysis of the intra- and inter-individual variability of the normal urinary proteome.
Nagarjuna Nagaraj,Matthias Mann +1 more
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TLDR
Determination of the normal fluctuation of individual urinary proteins should be useful in establishing significance thresholds in biomarker studies and allowed definition of a common and abundant set of 500 proteins that were readily detectable in all studied individuals.Abstract:
Urine is a readily and noninvasively obtainable body fluid. Mass spectrometry (MS)-based proteomics has shown that urine contains thousands of proteins. Urine is a potential source of biomarkers for diseases of proximal and distal tissues but it is thought to be more variable than the more commonly used plasma. By LC-MS/MS analysis on an LTQ-Orbitrap without prefractionation we characterized the urinary proteome of seven normal human donors over three consecutive days. Label-free quantification of triplicate single runs covered the urinary proteome to a depth of more than 600 proteins. The median coefficient of variation (cv) of technical replicates was 0.18. Interday variability was markedly higher with a cv of 0.48 and the overall variation of the urinary proteome between individuals was 0.66. Thus technical variability in our data was 7.5%, whereas intrapersonal variability contributed 45.5% and interpersonal variability contributed 47.1% to total variability. Determination of the normal fluctuation of individual urinary proteins should be useful in establishing significance thresholds in biomarker studies. Our data also allowed definition of a common and abundant set of 500 proteins that were readily detectable in all studied individuals. This core urinary proteome has a high proportion of secreted, membrane, and relatively high-molecular weight proteins.read more
Citations
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Journal ArticleDOI
Proteome Analysis of Urinary Biomarkers in Acute Hypercoagulable State Rat Model.
Jian Jing,Zhenhuan Du,Qin Weiwei +2 more
TL;DR: In this paper, human homologous proteins of differential proteins were found to be highly expressed in 31 human tissues, especially in the kidneys followed by digestive system and reproductive system, which may serve as potential biomarkers for the prevention and treatment of thrombotic diseases.
Book ChapterDOI
Effects of diuretics on urinary proteins.
TL;DR: The results suggest that the effects of diuretics deserve more attention in future urinary protein biomarker studies, and the distinct effects ofdiuretics on the urinary proteome may provide clues to the mechanisms of diUREtics.
Posted ContentDOI
Comparison of urine proteomes from tumor-bearing mice with those from tumor-resected mice
TL;DR: In this paper , changes in the urine proteome between tumor-bearing mice and tumor-resected mice were explored by using liquid chromatography coupled with tandem mass spectrometry (LCMS/MS).
Journal ArticleDOI
Constant urinary proteins in healthy humans in a 520-day isolation experiment
L. Kh. Pastushkova,K. S. Kireev,K. S. Kireev,A. S. Kononikhin,E. S. Tiys,Igor Popov,I. V. Dobrokhotov,Marc-Antoine Custaud,Vladimir A. Ivanisenko,Nikolay A. Kolchanov,E. N. Nikolaev,V. I. Pochuev,Irina M. Larina +12 more
TL;DR: Seven permanent proteins of the urinary proteome were identified and observed during the entire period of urine investigations: epidermal growth factor, polymeric immunoglobulin receptor, plasma serine protease inhibitor, Alpha 1 microglobulin/bikunin precursor (AMBP), keratin, collagen alpha-1 (VI) chain, and serum albumin.
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Ruedi Aebersold,Matthias Mann +1 more
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Universal sample preparation method for proteome analysis
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