Rapamycin (ay-22, 989), a new antifungal antibiotic i. taxonomy of the producing streptomycete and isolation of the active principle
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TLDR
From its characteristic ultraviolet absorption spectrum rapamycin can be classified as a triene and its activity is compared with that of amphotericin B, candicidin and nystatin.Abstract:
A streptomycete was isolated from an Easter Island soil sample and found to inhibit Candida albicans, Microsporum gypseum and Trichophyton granulosum. The antibiotic-producing microorganism was characterized and identified as Streptomyces hygroscopicus. The antifungal principle was extracted with organic solvent from the mycelium, isolated in crystalline form and named rapamycin. Rapamycin is mainly active against Candida albicans; minimum inhibitory concentration against ten strains ranged from 0.02 to 0.2 mug/ml. Its apparent activity against Microsporum gypseum and Trichophyton granulosum is lower because of its instability in culture media on prolonged incubation required by these fungi. No activity was observed against gram-positive and gram-negative bacteria. Acute toxicity in mice is low.read more
Citations
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Rapamycin, a potent inhibitor of T-cell function, prevents graft rejection in murine recipients of allogeneic T-cell-depleted donor marrow.
TL;DR: It is concluded that RAPA represents a new therapeutic modality for promoting alloengraftment and accelerating hematopoietic recovery after BM transplantation and is multilineage and durable.
Journal ArticleDOI
Acute systemic rapamycin induces neurobehavioral alterations in rats
Martin Hadamitzky,Arne Herring,Kathy Keyvani,Raphael Doenlen,Ute Krügel,Katharina Bösche,Kathrin Orlowski,Harald Engler,Manfred Schedlowski +8 more
TL;DR: It is demonstrated that acute blockade of mTOR signaling by acute rapamycin administration not only causes changes in neuronal activity, but also leads to elevated protein expression in protein kinase pathways others than mTOR, contributing to the development of anxiety-like behavior.
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Safety and pharmacokinetics of ascending single doses of sirolimus (Rapamune, rapamycin) in pediatric patients with stable chronic renal failure undergoing dialysis.
Amir Tejani,Steven R. Alexander,Robert B. Ettenger,Gary Lerner,James Zimmerman,Edward C. Kohaut,David M. Briscoe +6 more
TL;DR: It is suggested that younger pediatric patients might require an increased maintenance dose of sirolimus to achieve whole blood exposures similar to those in healthy adults, and the drug's tolerance, safety, and pharmacokinetic parameters in pediatric patients are reviewed.
Patent
Rapamycin polymorphs and uses thereof
Tianmin Zhu,Fawzi Mahdi B +1 more
TL;DR: In this article, the authors present a process for preparing rapamycin polymorph Form II and pharmaceutical compositions including Rapamycin polyform Form II, including Rapamyrin and Rabin.
Journal ArticleDOI
Biotic acts of antibiotics
TL;DR: Antibiotics can be effective in treatment of a broad spectrum of diseases and pathological conditions other than those of infectious etiology and, in this capacity, may find widespread applications beyond the intended antimicrobial use.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A simple method of estimating fifty per cent endpoints
Lowell J. Reed,H. Muench +1 more
Journal ArticleDOI
Methods for characterization of Streptomyces species
E. B. Shirling,D. Gottlieb +1 more
TL;DR: The methods used by collaborators in the ISP for emendation of descriptions of type and neotype strains of the genus Streptomyces (Actinomycetales) are presented.
Journal ArticleDOI
Simplified, Accurate Method for Antibiotic Assay of Clinical Specimens
TL;DR: Serum specimens are tested directly rather than diluting them to a narrow range of antibiotic concentrations, possible because of a procedure for calculations that recognizes the curvilinear relationship between zone sizes and antibiotic concentrations.