Rapamycin (ay-22, 989), a new antifungal antibiotic i. taxonomy of the producing streptomycete and isolation of the active principle
Reads0
Chats0
TLDR
From its characteristic ultraviolet absorption spectrum rapamycin can be classified as a triene and its activity is compared with that of amphotericin B, candicidin and nystatin.Abstract:
A streptomycete was isolated from an Easter Island soil sample and found to inhibit Candida albicans, Microsporum gypseum and Trichophyton granulosum. The antibiotic-producing microorganism was characterized and identified as Streptomyces hygroscopicus. The antifungal principle was extracted with organic solvent from the mycelium, isolated in crystalline form and named rapamycin. Rapamycin is mainly active against Candida albicans; minimum inhibitory concentration against ten strains ranged from 0.02 to 0.2 mug/ml. Its apparent activity against Microsporum gypseum and Trichophyton granulosum is lower because of its instability in culture media on prolonged incubation required by these fungi. No activity was observed against gram-positive and gram-negative bacteria. Acute toxicity in mice is low.read more
Citations
More filters
Journal ArticleDOI
Efficacy of sirolimus (rapamycin) administered concomitantly with a subtherapeutic dose of cyclosporin in the treatment of severe psoriasis: a randomized controlled trial.
TL;DR: The concomitant administration of sirolimus with a subtherapeutic dose of cyclosporin in severe psoriasis may permit a reduction in their respective toxicities, notably cyclosporain-induced nephrotoxicity.
Book ChapterDOI
Targeting the Metabolic Microenvironment of Tumors
TL;DR: A comprehensive summary of preclinical and clinical drugs under development for targeting aerobic glycolysis, acidosis, hypoxia and Hypoxia response pathways is provided, providing further therapeutic benefit for cancers that feature these common phenotypes.
Journal ArticleDOI
Liver damage, inflammation, and enhanced tumorigenesis after persistent mTORC1 inhibition.
Atsushi Umemura,Eek Joong Park,Koji Taniguchi,Jun Hee Lee,Shabnam Shalapour,Mark A. Valasek,Mariam Aghajan,Hayato Nakagawa,Hayato Nakagawa,Ekihiro Seki,Michael N. Hall,Michael Karin +11 more
TL;DR: Results suggest that long-term rapamycin treatment, which also increases IL-6 production in humans, is unsuitable for prevention or treatment of obesity-promoted liver cancer.
Journal ArticleDOI
Roles of mTOR complexes in the kidney: implications for renal disease and transplantation.
TL;DR: Clinical studies of mTOR pathway targeting will enable testing of evolving hypotheses and improve understanding of the complex effects of m TOR targeting on immune responses, including those that impact both de novo renal disease and renal allograft outcomes.
Journal ArticleDOI
A Predictive Model for Selective Targeting of the Warburg Effect through GAPDH Inhibition with a Natural Product
Maria V. Liberti,Maria V. Liberti,Ziwei Dai,Suzanne E. Wardell,Joshua A. Baccile,Xiaojing Liu,Xia Gao,Robert Baldi,Mahya Mehrmohamadi,Mahya Mehrmohamadi,Marc O. Johnson,Neel Madhukar,Alexander A. Shestov,Iok In Christine Chio,Olivier Elemento,Jeffrey C. Rathmell,Frank C. Schroeder,Donald P. McDonnell,Jason W. Locasale +18 more
TL;DR: It is shown that differences in rate-controlling enzymes during the Warburg effect (THE AUTHORS), the most prominent hallmark of cancer cell metabolism, can be used to predict a response to targeting glucose metabolism, and establishes a natural product, koningic acid, to be a selective inhibitor of GAPDH.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A simple method of estimating fifty per cent endpoints
Lowell J. Reed,H. Muench +1 more
Journal ArticleDOI
Methods for characterization of Streptomyces species
E. B. Shirling,D. Gottlieb +1 more
TL;DR: The methods used by collaborators in the ISP for emendation of descriptions of type and neotype strains of the genus Streptomyces (Actinomycetales) are presented.
Journal ArticleDOI
Simplified, Accurate Method for Antibiotic Assay of Clinical Specimens
TL;DR: Serum specimens are tested directly rather than diluting them to a narrow range of antibiotic concentrations, possible because of a procedure for calculations that recognizes the curvilinear relationship between zone sizes and antibiotic concentrations.