Red-shifted luciferase–luciferin pairs for enhanced bioluminescence imaging
Hsien-Wei Yeh,Omran Karmach,Ao Ji,David Carter,Manuela Martins-Green,Hui-wang Ai,Hui-wang Ai +6 more
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TLDR
The development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence are described and one pair showed superior in vitro and in vivo sensitivity over commonly used biolumeinescence reporters is adapted.Abstract:
Red-shifted bioluminescence reporters are desirable for biological imaging. We describe the development of red-shifted luciferins based on synthetic coelenterazine analogs and corresponding mutants of NanoLuc that enable bright bioluminescence. One pair in particular showed superior in vitro and in vivo sensitivity over commonly used bioluminescence reporters. We adapted this pair to develop a bioluminescence resonance-energy-based Antares reporter called Antares2, which offers improved signal from deep tissues.read more
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Multifunctional materials for implantable and wearable photonic healthcare devices.
Geon-Hui Lee,Geon-Hui Lee,Hanul Moon,Hanul Moon,Hyemin Kim,Gae Hwang Lee,Gae Hwang Lee,Woosung Kwon,Seunghyup Yoo,David Myung,Seok Hyun Yun,Zhenan Bao,Sei Kwang Hahn,Sei Kwang Hahn +13 more
TL;DR: This Review describes emerging multifunctional materials critical to the advent of next-generation implantable and wearable photonic healthcare devices and discusses the path for their clinical translation, along with the future research directions for the field, particularly regarding mobile healthcare and personalized medicine.
Journal ArticleDOI
Single-cell bioluminescence imaging of deep tissue in freely moving animals
Satoshi Iwano,Mayu Sugiyama,Hiroshi Hama,Akiya Watakabe,Naomi Hasegawa,Takahiro Kuchimaru,Kazumasa Z. Tanaka,Megumu Takahashi,Yoko Ishida,Jun-ichi Hata,Satoshi Shimozono,Kana Namiki,Takashi Fukano,Masahiro Kiyama,Hideyuki Okano,Shinae Kizaka-Kondoh,Thomas J. McHugh,Tetsuo Yamamori,Hiroyuki Hioki,Shojiro Maki,Atsushi Miyawaki +20 more
TL;DR: AkaBLI produced emissions in vivo that were brighter by a factor of 100 to 1000 than conventional systems, allowing noninvasive visualization of single cells deep inside freely moving animals, and is therefore a bioengineered light source to spur unprecedented scientific, medical, and industrial applications.
Journal ArticleDOI
De novo design of modular and tunable protein biosensors.
Alfredo Quijano-Rubio,Hsien-Wei Yeh,Jooyoung Park,Hansol Lee,Robert A. Langan,Scott E. Boyken,Marc J. Lajoie,Longxing Cao,Cameron M. Chow,Marcos C. Miranda,Jimin Wi,Hyo Jeong Hong,Lance Stewart,Byung-Ha Oh,Byung-Ha Oh,David Baker +15 more
TL;DR: In this paper, a de novo-designed protein-based biosensors are presented, which can be used to detect proteins such as SARS-CoV-2 and anti-SARS-antibodies.
Journal ArticleDOI
Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors
Hsien-Wei Yeh,Hui-wang Ai +1 more
TL;DR: This review first introduces the development of bioluminescent reporters, and next, their applications in sensing biological changes in vitro and in vivo as biosensors, and discusses chemiluminescence sensors that produce photons in the absence of luciferases.
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Molecular Probes for Autofluorescence-Free Optical Imaging.
Yuyan Jiang,Kanyi Pu +1 more
TL;DR: In this paper, the authors focus on the advancements of autofluorescence-free molecular probes through the lens of particular molecular or photophysical mechanisms that produce long-lasting luminescence after the cessation of light excitation.
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Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate.
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