Role of the snRNAs in spliceosomal active site
TLDR
Whether the spliceosome is an RNA or RNA-protein catalyst remains uncertain, as discovery of an RNase H-like domain in a splicedosomal protein that is closely associated with splice sites suggests that proteins may be involved in formation of the active site.Abstract:
The spliceosome, the ribonucleoprotein assembly that removes the intervening sequences from pre-mRNAs through splicing, is one of the most complex cellular machines. In human it is composed of ~150 proteins and five RNAs (snRNAs). One of the snRNAs, U6, contains sequences analogous to all the RNA elements that form the active site of the group II introns, ribozymes that perform a splicing reaction mechanistically identical to spliceosomal splicing. Interestingly, U6 is the only snRNA that is indispensable for splicing and in vitro, in complex with another snRNA, it can catalyze a primordial splicing reaction in the absence of all other spliceosomal factors. On the other hand, discovery of an RNase H-like domain in a spliceosomal protein that is closely associated with splice sites suggests that proteins may be involved in formation of the active site. Thus, whether the spliceosome is an RNA or RNA-protein catalyst remains uncertain.read more
Citations
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Journal ArticleDOI
A day in the life of the spliceosome
A. Gregory Matera,Zefeng Wang +1 more
TL;DR: Insights into these questions have been gained by studying the life cycle of spliceosomal snRNAs from their transcription, nuclear export and re-import to their dynamic assembly into thespliceosome.
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A small noncoding RNA signature found in exosomes of GBM patient serum as a diagnostic tool
Lorea Manterola,Elizabeth Guruceaga,Jaime Gállego Pérez-Larraya,Marisol Gonzalez-Huarriz,Patricia Jauregui,Sonia Tejada,Ricardo Díez-Valle,Victor Segura,Nicolás Samprón,Cristina Barrena,Irune Ruiz,Amaia Agirre,Angel Ayuso,Javier Rodríguez,Alvaro Gonzalez,Enric Xipell,Ander Matheu,Adolfo López de Munain,Teresa Tuñón,Idoya Zazpe,Jesus Garcia-Foncillas,Sophie Paris,Jean Yves Delattre,Marta M. Alonso +23 more
TL;DR: A small noncoding RNA signature in microvesicles isolated from GBM patient serum is uncovered that could be used as a fast and reliable differential diagnostic biomarker.
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Pick one, but be quick: 5′ splice sites and the problems of too many choices
TL;DR: The history of research on 5' Splice sites selection is reviewed, highlighting the difficulties of establishing how base-pairing strength determines splicing outcomes and proposed that protein complexes propagate along the exon, thereby changing its physical behavior so as to affect 5'ss selection.
Patent
Cancer-related biological materials in microvesicles
TL;DR: In this article, methods for assaying a biological sample from a subject by analyzing components of microvesicle fractions in aid of risk, diagnosis, prognosis or monitoring of, or directing treatment of the subject for, a disease or other medical condition in the subject.
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Small RNAs derived from longer non-coding RNAs
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TL;DR: This review will focus on several recent findings that have added new aspects to small RNA-guided gene silencing in mediating transposon silencing.
References
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Journal ArticleDOI
The Spliceosome: Design Principles of a Dynamic RNP Machine
TL;DR: The spliceosome exhibits exceptional compositional and structural dynamics that are exploited during substrate-dependent complex assembly, catalytic activation, and active site remodeling in the pre-mRNAs.
Journal ArticleDOI
The evolution of spliceosomal introns: patterns, puzzles and progress.
Scott William Roy,Walter Gilbert +1 more
TL;DR: Patterns of intron-position correspondence between widely diverged eukaryotic species have provided insights into the origins of the vast differences in intron number between eukARYotic species, and studies of specific cases of introns loss and gain have led to progress in understanding the underlying molecular mechanisms and the forces that control intron evolution.
Journal ArticleDOI
Crystal structure of a self-spliced group II intron.
TL;DR: Structural and functional analogies support the hypothesis that group II introns and the spliceosome share a common ancestor.