Screening and optimization of protease production from a halotolerant Bacillus licheniformis isolated from saltern sediments
Chandrasekaran Suganthi,Anbazhagan Mageswari,S. Karthikeyan,M. Anbalagan,A. Sivakumar,Kodiveri Muthukaliannan Gothandam +5 more
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TLDR
Studies on the effect of different carbon and nitrogen sources revealed that xylose and urea enhances the enzyme production, and with selected C–N sources along with 1 M NaCl the maximum protease production was obtained.About:
This article is published in Journal of Genetic Engineering and Biotechnology.The article was published on 2013-06-01 and is currently open access. It has received 67 citations till now. The article focuses on the topics: Bacillus licheniformis & Protease.read more
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Development and application of a CRISPR/Cas9 system for Bacillus licheniformis genome editing.
Cuixia Zhou,Huan Liu,Feiyan Yuan,Haonan Chai,Haikuan Wang,Fufeng Liu,Yu Li,Huitu Zhang,Fuping Lu +8 more
TL;DR: The results illustrated that the establishment of a CRISPR/Cas9 system for Bacillus licheniformis is of significance, and confirmed the system's high efficiency, and offers promise for applications in genetic modification of other industrial relevant Bacillus species.
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Development and characterization of cross-linked enzyme aggregates of thermotolerant alkaline protease from Bacillus licheniformis.
TL;DR: It was concluded that immobilized enzyme in the form of CLEAs is a valuable catalyst for potential industrial applications and thermo-tolerant with optimum activity at 65°C temperature.
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Improved β-amylase production on molasses and sugar beet pulp by a novel strain Paenibacillus chitinolyticus CKS1
Katarina Mihajlovski,Neda Radovanović,Đorđe Veljović,Slavica Šiler-Marinković,Suzana Dimitrijević-Branković +4 more
TL;DR: In this article, a new strain Paenibacillus chitinolyticus CKS1 was performed using a molasses and sugar beet pulp, and a statistical model was designed via Central Composite Design (CCD) under the Response Surface Methodology (RSM) for obtaining maximum β-amylase activity.
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Screening, isolation and characterization of protease producing moderately halophilic microorganism Halomonas meridiana associated with coral mucus
TL;DR: Starch and beef extract were the best substrates for enzyme production and studies on enzymatic characterization revealed that crude protease showed maximal activity at pH 7 and 37°C, which indicates the enzyme to be normal or moderately alkaline.
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The Bacteriology and Its Virulence Factors in Neonatal Infections: Threats to Child Survival Strategies
Obiora Shedrach Ejiofor,Onyinye Mercy Ajunwa,Chijioke Elias Ezeudu,George Ogonna Emechebe,Kenneth Nchekwube Okeke,Christian Chukwuemeka Ifezulike,IM Ekejindu,Jude Nnaemeka Okoyeh,Eunice Ogonna Osuala,Angus Nnamdi Oli +9 more
TL;DR: Pseudomonas spp, Escherichia coli, Salmonella spp.
References
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Biology of moderately halophilic aerobic bacteria
TL;DR: The moderately halophilic heterotrophic aerobic bacteria form a diverse group of microorganisms and approaches to the study of genetic processes have recently been developed, opening the way toward an understanding of haloadaptation at the molecular level.
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Improvement of microbial strains and fermentation processes.
TL;DR: This review focuses on the various options that may be employed to improve microbial strains and addresses the complex problems of screening, the tools and technology behind the selection of targeted organisms, and the importance of process optimization.
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Complete genome sequence of the industrial bacterium Bacillus licheniformis and comparisons with closely related Bacillus species
Michael Rey,Preethi Ramaiya,Beth Nelson,Shari D Brody-Karpin,Elizabeth Zaretsky,Maria Tang,Alfredo Lopez de Leon,Henry Xiang,Veronica Gusti,Ib Groth Clausen,Ib Groth Clausen,Peter Bjarke Olsen,Michael Dolberg Rasmussen,Jens Tonne Andersen,Per Linå Jørgensen,Thomas Schou Larsen,Alexei Sorokin,Alexander Bolotin,Alla Lapidus,Alla Lapidus,Nathalie Galleron,S. Dusko Ehrlich,Randy M. Berka +22 more
TL;DR: Despite the unmistakable organizational similarities between the B. licheniformis and B. subtilis genomes, there are notable differences in the numbers and locations of prophages, transposable elements and a number of extracellular enzymes and secondary metabolic pathway operons that distinguish these species.
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Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches.
TL;DR: The production of alkaline protease was optimized using a newly isolated Bacillus sp.
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Extracellular alkaline protease from a newly isolated haloalkaliphilic Bacillus sp.: Production and optimization
TL;DR: A newly isolated haloalkaliphilic Bacillus sp.