Serological profiles of pan-coronavirus-specific responses in COVID-19 patients using a multiplexed electro-chemiluminescence-based testing platform.
Sidhartha Chaudhury,Jack N Hutter,Jessica S Bolton,Shilpa Hakre,Evelyn Mose,Amy I Wooten,William D O'Connell,Joseph Hudak,Shelly J. Krebs,Janice Darden,Jason A. Regules,Clinton K. Murray,Kayvon Modjarrad,Paul T. Scott,Sheila A. Peel,Elke S. Bergmann-Leitner +15 more
Reads0
Chats0
TLDR
In this article, a 10-plex electro-chemiluminescence-based method was used to measure IgM and IgG responses to the spike proteins from multiple human coronaviruses including SARS-CoV-2.Citations
More filters
Journal ArticleDOI
SARS-CoV-2 vaccination elicits unconventional IgM specific responses in naïve and previously COVID-19-infected individuals
Alessandra Ruggiero,Chiara Piubelli,Lucia Calciano,Simone Accordini,Maria Teresa Valenti,Luca Dalle Carbonare,Gabriel Siracusano,Nigel J. Temperton,Natalia Tiberti,Silvia S. Longoni,Massimo Pizzato,Silvia Accordini,Tobia Fantoni,Lucia Lopalco,Alberto Beretta,Zeno Bisoffi,Donato Zipeto +16 more
TL;DR: In this article , a longitudinal study was performed to quantify anti-S SARS-CoV-2 IgG and IgM (IgG-S and IgM-S) in health care worker (HCW) recipients of the BNT162b2 vaccine.
Journal ArticleDOI
Mapping SARS-CoV-2 Antibody Epitopes in COVID-19 Patients with a Multi-Coronavirus Protein Microarray.
David Camerini,Arlo Randall,Krista Trappl-Kimmons,Amit Oberai,Christopher Hung,Joshua Edgar,Adam D. Shandling,Vu T. Huynh,Andy Teng,Gary Hermanson,Jozelyn Pablo,Megan M Stumpf,Sandra Lester,Jennifer L Harcourt,Azaibi Tamin,Mohammed Ata Ur Rasheed,Natalie J. Thornburg,Panayampalli Subbian Satheshkumar,Xiaowu Liang,Richard B. Kennedy,Angela Yee,Michael B. Townsend,Joseph J. Campo +22 more
TL;DR: In this article, a multi-coronavirus protein microarray was created containing full-length proteins, overlapping protein fragments of various lengths, and peptide libraries from SARS-CoV-2 and four other human coronaviruses.
Journal ArticleDOI
Possible Targets of Pan-Coronavirus Antiviral Strategies for Emerging or Re-Emerging Coronaviruses.
TL;DR: In this article, the authors discuss the latest progress of possible targets of pan-coronavirus antiviral strategies for emerging or re-emerging coronaviruses, including targets for pan-caravirus inhibitors and vaccines, which will provide prospects for the current and future research and treatment of the disease.
Journal ArticleDOI
Comprehensive serologic profile and specificity of maternal and neonatal cord blood SARS-CoV-2 antibodies
TL;DR: In this paper , the profile and specificity of maternal and neonatal cord blood antibody profiles in response to SARS-CoV-2 virus exposure were described in a prospective cohort study of delivering patients at Thomas Jefferson University Hospital from April 2020 to February 2021.
Journal ArticleDOI
IgM responses following SARS-CoV-2 vaccination: insights into protective and pre-existing immunity
TL;DR: Ruggiero et al. as discussed by the authors performed a longitudinal study of a large cohort of health care workers to study the dynamic IgM response following BNT162b2 vaccination in naïve and previously infected individuals with SARS-CoV-2.
References
More filters
Posted ContentDOI
Prevalence of IgG antibodies to SARS-CoV-2 in Wuhan – Implications for the ability to produce long-lasting protective antibodies against SARS-CoV-2
TL;DR: Very few healthcare providers had IgG antibodies to SARS-CoV-2, though a significant proportion of them had been infected with the virus, and people are unlikely to produce long-lasting protective antibodies against this virus.
Journal ArticleDOI
A High-Throughput Assay for Circulating Antibodies Directed Against the S Protein of Severe Acute Respiratory Syndrome Coronavirus 2.
Svenja Weiss,Jéromine Klingler,Catarina E. Hioe,Catarina E. Hioe,Fatima Amanat,Ian Baine,Suzanne Arinsburg,Erna Milunka Kojic,Jonathan Stoever,Sean T. H. Liu,Denise Jurczyszak,Maria Bermudez-Gonzalez,Viviana Simon,Florian Krammer,Susan Zolla-Pazner +14 more
TL;DR: A Luminex binding assay was developed and used to assess simultaneously the presence of coronavirus disease 2019 (COVID-19)–specific antibodies in human serum and plasma, and clear differentiation was achieved between specimens from infected and uninfected subjects, and a wide range of serum/plasma antibody levels was delineated in infected subjects.
Journal ArticleDOI
Comparison of ELISA with electro-chemiluminescence technology for the qualitative and quantitative assessment of serological responses to vaccination.
Jessica S. Bolton,Sidhartha Chaudhury,Sheetij Dutta,Scott Gregory,Emily Locke,Tony Pierson,Elke S. Bergmann-Leitner +6 more
TL;DR: The advantages of the newly developed tool for assessing the antigen profiles of serological responses may ultimately lead to the identification of biomarkers associated with various disease stages and or protection against disease.
Journal ArticleDOI
Breadth of humoral immune responses to the C-terminus of the circumsporozoite protein is associated with protective efficacy induced by the RTS,S malaria vaccine.
Sidhartha Chaudhury,Randall S. MacGill,Angela M. Early,Jessica S. Bolton,C. Richter King,Emily Locke,Tony Pierson,Dyann F. Wirth,Daniel E. Neafsey,Elke S. Bergmann-Leitner +9 more
TL;DR: In this paper, the authors measured the breadth of C-terminal antibody responses against CSP-specific antigenic variants and found that responses to the variants showed, on average, a 30-fold reduction in reactivity relative to the vaccine-matched 3D7 allele.