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“Sight‐unseen” detection of rare aquatic species using environmental DNA

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TLDR
Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.
Abstract
Effective management of rare species, including endangered native species and recently introduced nonindigenous species, requires the detection of populations at low density. For endangered species, detecting the localized distribution makes it possible to identify and protect critical habitat to enhance survival or reproductive success. Similarly, early detection of an incipient invasion by a harmful species increases the feasibility of rapid responses to eradicate the species or contain its spread. Here we demonstrate the efficacy of environmental DNA (eDNA) as a detection tool in freshwater environments. Specifically, we delimit the invasion fronts of two species of Asian carps in Chicago, Illinois, USA area canals and waterways. Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.

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Hydrologic monitoring and selected hydrologic and environmental studies by the U.S. Geological Survey in Georgia, 2011–2013

Abstract: The U.S. Geological Survey (USGS) has been requested by the U.S. Army Corps of Engineers (USACE)-Savannah District to install and operate a hydrologic and water-quality monitoring network within the Savannah River estuary starting in October 2013 in support of the expected Savannah Harbor Expansion Project (SHEP). The intent of the SHEP is to deepen the shipping channel by 5 feet to a new depth of 47 feet, which would allow super-cargo container ships to use the Port of Savannah. After years of monitoring and analyses by a diverse group of Federal, State, and local stakeholders, the primary concern about the project is the consequent potential for increased salinity encroachment, which may damage fragile ecosystems, threaten public water intakes, and decrease the dissolved oxygen levels within the Savannah River. The SHEP hydrologic and water-quality monitoring network would involve the installation of five new stations with tidal discharge and continuous water-quality instruments and the upgrade of eight existing stations to an equivalent data instrument array (fig. 1). The hydrologic monitoring is designed to occur in three phases of channel construction: a 1-year pre-construction phase, a 4-year construction phase, and a 10-year post-construction phase. A subset of the proposed locations would continue to be operated well beyond the project timeframe. Additionally, a chloride warning network will be installed to protect the public drinking-water intakes in the upper Savannah River estuary. All data will be available in real-time on the USGS National Water Information System Web Interface (NWISWeb; http://waterdata. usgs.gov) and will also be provided to the SHEP database managed by the USACE for frequent modeling updates. 02198980 02198955 02198950 02198920 02198840 02198820 02198810
Posted ContentDOI

Experimental evaluation of environmental DNA detection of a rare fish in turbid water

TL;DR: In this paper , 1-L field-collected water samples were spiked with tank-sourced eDNA from a rare, endangered estuarine fish at concentrations similar to eDNA samples collected from the natural environment.
Journal ArticleDOI

A Geospatial Approach to Improving Fish Species Detection in Maumee Bay, Lake Erie

TL;DR: The U.S. Fish and Wildlife Service's Early Detection and Monitoring (EDM) program has been monitoring for new invasive species since 2013 and is continually looking to adapt sampling methods to improve efficiency to increase the chance of detecting new aquatic invasive species at low abundances as mentioned in this paper .
References
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Journal ArticleDOI

MEGA: A biologist-centric software for evolutionary analysis of DNA and protein sequences

TL;DR: The motivation, design principles and priorities that have shaped the development of MEGA are discussed and how MEGA might evolve in the future to assist researchers in their growing need to analyze large data set using new computational methods are discussed.
Journal ArticleDOI

The role of propagule pressure in explaining species invasions.

TL;DR: Propagule pressure is proposed as a key element to understanding why some introduced populations fail to establish whereas others succeed and how the study of propagule pressure can provide an opportunity to tie together disparate research agendas within invasion ecology.
Journal ArticleDOI

Species detection using environmental DNA from water samples.

TL;DR: A novel approach, based on the limited persistence of DNA in the environment, to detect the presence of a species in fresh water, using specific primers that amplify short mitochondrial DNA sequences to track the existence of a frog in controlled environments and natural wetlands.
Journal ArticleDOI

Biological invasions: recommendations for U.S. policy and management.

TL;DR: The Ecological Society of America recommends that the federal government take the following six actions: use new information and practices to better manage commercial and other pathways to reduce the transport and release of potentially harmful species, and establish a National Center for Invasive Species Management.
Journal ArticleDOI

An ounce of prevention or a pound of cure: bioeconomic risk analysis of invasive species

TL;DR: A quantitative bioeconomic modelling framework is presented to analyse risks from non–indigenous species to economic activity and the environment, and it is shown that society could benefit by spending up to US$324 000 year−1 to prevent invasions into a single lake with a power plant.
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