“Sight‐unseen” detection of rare aquatic species using environmental DNA
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TLDR
Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.Abstract:
Effective management of rare species, including endangered native species and recently introduced nonindigenous species, requires the detection of populations at low density. For endangered species, detecting the localized distribution makes it possible to identify and protect critical habitat to enhance survival or reproductive success. Similarly, early detection of an incipient invasion by a harmful species increases the feasibility of rapid responses to eradicate the species or contain its spread. Here we demonstrate the efficacy of environmental DNA (eDNA) as a detection tool in freshwater environments. Specifically, we delimit the invasion fronts of two species of Asian carps in Chicago, Illinois, USA area canals and waterways. Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.read more
Citations
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DNA (meta)barcoding of biological invasions: a powerful tool to elucidate invasion processes and help managing aliens
TL;DR: DNA barcoding, and its recent extension, DNA metabarcoding are complementary tools that have proved their value in the identification of living beings and how they can be applied in the control and management of biological introductions are reviewed.
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An environmental DNA-based method for monitoring spawning activity: a case study, using the endangered Macquarie perch (Macquaria australasica)
Jonas Bylemans,Jonas Bylemans,Elise M. Furlan,Elise M. Furlan,Christopher M. Hardy,Prudence McGuffie,Prudence McGuffie,Mark Lintermans,Dianne Gleeson,Dianne Gleeson +9 more
TL;DR: It is shown that changes in the relative abundance of nuclear and mitochondrial eDNA can be used to monitor spawning activity of the endangered Macquarie perch and is likely to be transferrable to other aquatic species.
Journal ArticleDOI
Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data.
TL;DR: Current monitoring programs must consider alternative vectors of DNA in the environment and consider alternative strategies to minimize the detection of DNA not directly released from live bigheaded carps.
Journal ArticleDOI
Environmental DNA (eDNA) metabarcoding assays to detect invasive invertebrate species in the Great Lakes.
TL;DR: Two metabarcoding eDNA assays using the mtDNA 16S RNA gene with Illumina MiSeq platform to detect invertebrate fauna in the Laurentian Great Lakes and surrounding waterways are developed, with a focus for use on invasive bivalve and gastropod species monitoring.
Journal ArticleDOI
Wanted dead or alive? Using metabarcoding of environmental DNA and RNA to distinguish living assemblages for biosecurity applications
Xavier Pochon,Anastasija Zaiko,Anastasija Zaiko,Anastasija Zaiko,Lauren M. Fletcher,Olivier Laroche,Susanna A. Wood,Susanna A. Wood +7 more
TL;DR: The data suggest that the presence of eRNA-only OTUs could be due to increased cellular activities of some rare taxa that were not identified in the eDNA datasets, unusually high numbers of rRNA transcripts in ciliates, and/or artefacts produced during the reverse transcriptase, PCR and sequencing steps.
References
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