“Sight‐unseen” detection of rare aquatic species using environmental DNA
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TLDR
Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.Abstract:
Effective management of rare species, including endangered native species and recently introduced nonindigenous species, requires the detection of populations at low density. For endangered species, detecting the localized distribution makes it possible to identify and protect critical habitat to enhance survival or reproductive success. Similarly, early detection of an incipient invasion by a harmful species increases the feasibility of rapid responses to eradicate the species or contain its spread. Here we demonstrate the efficacy of environmental DNA (eDNA) as a detection tool in freshwater environments. Specifically, we delimit the invasion fronts of two species of Asian carps in Chicago, Illinois, USA area canals and waterways. Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.read more
Citations
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Evaluating intraspecific genetic diversity of a fish population using environmental DNA: An approach to distinguish true haplotypes from erroneous sequences
TL;DR: The approach proposed in this study successfully eliminated most of false positive haplotypes in the HTS data obtained from eDNA samples, which allowed us to improve the detection accuracy for evaluating intraspecific diversity using eDNA analysis.
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Aquatic environmental DNA: A review of the macro-organismal biomonitoring revolution.
Miwa Takahashi,Mattia Sacco,Joshua H. Kestel,Georgia M. Nester,Matthew A. Campbell,M. van der Heyde,Matthew Heydenrych,David J. Juszkiewicz,Paul G. Nevill,Kathryn L. Dawkins,Cindy Bessey,Kristen Fernandes,Haylea C. Miller,Matthew Power,Mahsa Mousavi-Derazmahalleh,Joshua P. Newton,Nicole White,Zoe T. Richards,Morten E. Allentoft +18 more
TL;DR: A systematic literature review of 407 peer-reviewed papers on aquatic eDNA published between 2012 and 2021 is presented in this paper , showing a gradual increase in the annual number of publications from four (2012) to 28 (2018) followed by a rapid growth to 124 publications in 2021, which was mirrored by a tremendous diversification of methods in all aspects of the eDNA workflow.
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Detection of the endangered European weather loach ( Misgurnus fossilis) via water and sediment samples: Testing multiple eDNA workflows
Lena Maureen Kusanke,Jörn Panteleit,Stefan Stoll,Egbert Korte,Eike Sünger,Ralf Schulz,Kathrin Theissinger +6 more
TL;DR: The results showed that the long‐term water preservation method commonly used for eDNA surveys of M. fossilis did not lead to optimal DNA yields, and a cost‐efficient high salt DNA extraction led to the highest target DNA yields and can be used for sediment and water samples.
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Needles in a haystack: Genomic tools have become powerful tools for conservation biologists to monitor the spread of invasive species
TL;DR: The use of environmental DNA (eDNA) to detect R. catesbeiana without observing the animals themselves allows the reliable detection of secretive organisms in wetlands without direct observation, and opens new perspectives for the assessment of current biodiversity from environmental samples.
Journal ArticleDOI
Combining surface and soil environmental DNA with artificial cover objects to improve terrestrial reptile survey detection
Kathleen E. Kyle,Michael C. Allen,J. Dragon,John F. Bunnell,Howard K. Reinert,Robert T. Zappalorti,Benjamin D Jaffe,Jordan C. Angle,Julie L. Lockwood +8 more
TL;DR: In this paper , the authors tested the degree to which inclusion of surface and soil eDNA sampling into conventional artificial cover methods elevates the detection probability of a small, cryptic terrestrial lizard, Scincella lateralis.
References
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