“Sight‐unseen” detection of rare aquatic species using environmental DNA
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TLDR
Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.Abstract:
Effective management of rare species, including endangered native species and recently introduced nonindigenous species, requires the detection of populations at low density. For endangered species, detecting the localized distribution makes it possible to identify and protect critical habitat to enhance survival or reproductive success. Similarly, early detection of an incipient invasion by a harmful species increases the feasibility of rapid responses to eradicate the species or contain its spread. Here we demonstrate the efficacy of environmental DNA (eDNA) as a detection tool in freshwater environments. Specifically, we delimit the invasion fronts of two species of Asian carps in Chicago, Illinois, USA area canals and waterways. Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.read more
Citations
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Journal ArticleDOI
Effective monitoring of freshwater fish
Johannes Radinger,J. Robert Britton,Stephanie M. Carlson,Anne E. Magurran,Juan Diego Alcaraz-Hernández,Ana Almodóvar,Lluís Benejam,Carlos Fernández-Delgado,Graciela G. Nicola,Francisco J. Oliva-Paterna,Mar Torralva,Emili García-Berthou +11 more
TL;DR: In this paper, the authors highlight the importance of identifying the key aims in monitor- ing programs and outlines the different methods of sampling freshwater fish that can be used to meet these aims, emphasizing that investigators must address is- sues around sampling design, statistical power, species detectability, taxonomy and ethics in their monitoring programmes.
Journal ArticleDOI
Active and passive environmental DNA surveillance of aquatic invasive species
TL;DR: This study uses active (targeted) and passive molecular surveillance approaches to detect species in the Muskingum River Watershed in Ohio, USA using digital droplet polymerase chain reaction (ddPCR).
Journal ArticleDOI
Release and degradation of environmental DNA and RNA in a marine system.
Susanna A. Wood,Laura Biessy,Janie L. Latchford,Anastasija Zaiko,Ulla von Ammon,François Audrezet,Melania E. Cristescu,Xavier Pochon +7 more
TL;DR: The finding of the prolonged persistence of eRNA may provide new opportunities for improved biodiversity surveys through reducing false positives caused by legacy DNA and could also facilitate new research on environmental transcriptomics.
Journal ArticleDOI
Techniques for the practical collection of environmental DNA : filter selection, preservation, and extraction
TL;DR: It is proposed that the appropriate selection method for eDNA analysis should be chosen based on context, when a high concentration of the target DNA is expected, such as in an aquarium experiment, ethanol precipitation is advantageous; however, when the targetDNA is rare, filtration followed by freezing or DNA fixation by ethanol and phenol treatment are recommended.
Journal ArticleDOI
Experimental observations on the decay of environmental DNA from bighead and silver carps
Richard F. Lance,Katy E. Klymus,Catherine A. Richter,Xin Guan,Heather L. Farrington,Matthew R. Carr,Nathan Thompson,Duane C. Chapman,Kelly Baerwaldt +8 more
TL;DR: In this article, the authors explored decay of eDNA associated with bighead carp (Hypophthalmichthys nobilis) biological waste collected from an aquaculture filtration system and with sperm collected from captive silver carp (H. molitrix), and how decay may be influenced by differing levels of water turbulence, temperature, microbial load, and pH.
References
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