“Sight‐unseen” detection of rare aquatic species using environmental DNA
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TLDR
Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.Abstract:
Effective management of rare species, including endangered native species and recently introduced nonindigenous species, requires the detection of populations at low density. For endangered species, detecting the localized distribution makes it possible to identify and protect critical habitat to enhance survival or reproductive success. Similarly, early detection of an incipient invasion by a harmful species increases the feasibility of rapid responses to eradicate the species or contain its spread. Here we demonstrate the efficacy of environmental DNA (eDNA) as a detection tool in freshwater environments. Specifically, we delimit the invasion fronts of two species of Asian carps in Chicago, Illinois, USA area canals and waterways. Quantitative comparisons with traditional fisheries surveillance tools illustrate the greater sensitivity of eDNA and reveal that the risk of invasion to the Laurentian Great Lakes is imminent.read more
Citations
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Environmental DNA reveals tropical shark diversity in contrasting levels of anthropogenic impact
Judith Bakker,Owen S. Wangensteen,Demian D. Chapman,Germain Boussarie,Dayne Buddo,Tristan L. Guttridge,Heidi Hertler,David Mouillot,Laurent Vigliola,Stefano Mariani +9 more
TL;DR: It is demonstrated that eDNA metabarcoding can be effectively employed to study shark diversity and further developments in this field have the potential to drastically enhance the ability to assess and monitor elusive oceanic predators, and lead to improved conservation strategies.
Journal ArticleDOI
Environmental DNA (eDNA) From the Wake of the Whales: Droplet Digital PCR for Detection and Species Identification
TL;DR: Droplet digital (dd)PCR technology for detection and species identification of cetaceans using environmental (e)DNA collected from seawater is adopted, with a focus on identification of known killer whale ecotypes.
Journal ArticleDOI
A novel environmental DNA approach to quantify the cryptic invasion of non-native genotypes
TL;DR: This work developed a method to quantify the relative proportion of native and non‐native DNA based on a single‐nucleotide polymorphism using cycling probe technology in real‐time PCR and revealed a promising method for risk assessment and management in biodiversity conservation.
Journal ArticleDOI
Influence of Stream Bottom Substrate on Retention and Transport of Vertebrate Environmental DNA
Christopher L. Jerde,Christopher L. Jerde,Brett P. Olds,Brett P. Olds,Arial J. Shogren,Elizabeth A. Andruszkiewicz,Elizabeth A. Andruszkiewicz,Andrew R. Mahon,Diogo Bolster,Jennifer L. Tank +9 more
TL;DR: It is found that while ddPCR improves the sensitivity of detection, the residence time and transport of eDNA does not follow the same dynamics as the conservative tracer and necessitates a more stochastic framework for modeling eDNA transport.
Journal ArticleDOI
Invasive bighead and silver carp effects on zooplankton communities in the Illinois River, Illinois, USA
Greg G. Sass,Collin Hinz,Anthony C. Erickson,Nerissa N. McClelland,Michael A. McClelland,John M. Epifanio +5 more
TL;DR: The results suggest that bighead and silver carp have changed the zooplankton community of the Illinois River which may have implications for the food web, native species, and other ecosystems poised to be invaded, such as the Laurentian Great Lakes.
References
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