Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
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TLDR
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.About:
This article is published in Gene.The article was published on 1988-07-15. It has received 6003 citations till now. The article focuses on the topics: Fusion protein & Expression vector.read more
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Human and murine serine-palmitoyl-CoA transferase--cloning, expression and characterization of the key enzyme in sphingolipid synthesis.
Bertram Weiss,Wilhelm Stoffel +1 more
TL;DR: The results provide conclusive genetic and biochemical evidence that the human and murine LCB2 genes described here encode serine palmitoyltransferase, and indicate that cell lines transfected with mLCB2 preferentially use the excess sphingoid bases for glucocerebroside and galactocere Broside synthesis.
Journal ArticleDOI
Analysis of the actin-binding domain of alpha-actinin by mutagenesis and demonstration that dystrophin contains a functionally homologous domain.
TL;DR: The actin-binding site in alpha-actinin was defined and it was established that the NH2-terminal domain of dystrophin is functionally as well as structurally homologous to that inalpha- actinin.
Journal ArticleDOI
Genetic engineering of proteins with cell membrane permeability
TL;DR: A method to engineer proteins with cell-membrane permeability by efficiently importing GST-MTS fusion proteins into NIH 3T3 fibroblasts and other cells is reported.
Patent
Soluble hyaluronidase glycoprotein (shasegp), process for preparing the same, uses and pharmaceutical compositions comprising thereof
TL;DR: In this article, a soluble neutral active hyaluronidase glycoproteins (sHASEGP's) were described and methods of manufacture and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies.
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Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Journal ArticleDOI
Determination of protein: A modification of the lowry method that gives a linear photometric response
TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
Journal ArticleDOI
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA
Ellson Y. Chen,Peter H. Seeburg +1 more
TL;DR: A method for obtaining sequence information directly from plasmid DNA is presented, and the advantages include speed, simplicity, avoidance of additional cloning steps into single-stranded phage M13 vectors, and hence applicability to sequencing large numbers of samples.
Journal ArticleDOI
Efficient isolation of genes by using antibody probes.
Richard A. Young,Ronald W. Davis +1 more
TL;DR: A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.