Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
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TLDR
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.About:
This article is published in Gene.The article was published on 1988-07-15. It has received 6003 citations till now. The article focuses on the topics: Fusion protein & Expression vector.read more
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p21CIP1-mediated inhibition of cell proliferation by overexpression of the gax homeodomain gene.
Roy C. Smith,D. Branellec,David H. Gorski,Kun Guo,Harris Perlman,Jean François Dedieu,Christopher Pastore,Abderrahim Mahfoudi,Patrice Denefle,Jeffrey M. Isner,Kenneth Walsh +10 more
TL;DR: Data indicate that gax overexpression can inhibit cell proliferation in a p21-dependent manner and can modulate injury-induced changes in vessel wall morphology that result from excessive cellular proliferation.
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The iron transporter Fth1p forms a complex with the Fet5 iron oxidase and resides on the vacuolar membrane.
TL;DR: The iron transporter homologue Fth1p is characterized, which is similar to the high affinity plasma membrane iron transporter Ftr1p and was localized to the vacuolar surface and did not undergo Pep4-dependent degradation.
Journal Article
Differential in Vivo and in Vitro Expression of Vascular Endothelial Growth Factor (VEGF)-C and VEGF-D in Tumors and Its Relationship to Lymphatic Metastasis in Immunocompetent Rats
Jaya Krishnan,Vladimir Kirkin,Anja Steffen,Martin Hegen,Debbie Weih,Stanislav I. Tomarev,Jörg Wilting,Jonathan P. Sleeman +7 more
TL;DR: It is shown that expression of VEGF-C and V EGF-D in tissue culture does not reflect expression in vivo and that activation of VegFR-3 in the absence of V EGFR-2 activation is sufficient to promote tumor-induced lymphangiogenesis and metastasis, and they support the notion that blockade of VECF-3 activation will be useful as a novel form of cancer therapy.
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Oligohis-tags: mechanisms of binding to Ni2+-NTA surfaces.
TL;DR: The binding mechanism of His‐tags to Ni2+‐NTA was investigated and it was demonstrated that two His separated by either one or four residues are the preferred binding motifs within hexahis tag, and elongation of these referred motifs decreased affinity.
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Negative regulation of the weel protein kinase by direct action of the nim1/cdr1 mitotic inducer
TL;DR: Direct biochemical evidence is provided that the wee1 protein is subject to negative regulation by phosphorylation and indicate that the nim1 protein acts as an inhibitory, wee1-specific kinase.
References
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Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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Determination of protein: A modification of the lowry method that gives a linear photometric response
TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
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Supercoil sequencing: a fast and simple method for sequencing plasmid DNA
Ellson Y. Chen,Peter H. Seeburg +1 more
TL;DR: A method for obtaining sequence information directly from plasmid DNA is presented, and the advantages include speed, simplicity, avoidance of additional cloning steps into single-stranded phage M13 vectors, and hence applicability to sequencing large numbers of samples.
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Efficient isolation of genes by using antibody probes.
Richard A. Young,Ronald W. Davis +1 more
TL;DR: A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.