Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
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TLDR
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.About:
This article is published in Gene.The article was published on 1988-07-15. It has received 6003 citations till now. The article focuses on the topics: Fusion protein & Expression vector.read more
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Neurexins: synaptic cell surface proteins related to the alpha-latrotoxin receptor and laminin.
Yuri A. Ushkaryov,Alexander G. Petrenko,Alexander G. Petrenko,Martin Geppert,Martin Geppert,Thomas C. Südhof,Thomas C. Südhof +6 more
TL;DR: The polymorphic structure of the neurexins, their neural localization, and their sequence similarity to proteins associated with neurogenesis suggest a function as cell recognition molecules in the nerve terminal.
Journal ArticleDOI
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element.
Shaorong Chong,Fana B. Mersha,Donald G. Comb,Melissa E. Scott,David Landry,Luis M. Vence,Francine B. Perler,Jack S. Benner,Rebecca Kucera,Christine A. Hirvonen,John J Pelletier,Henry Paulus,Ming Qun Xu +12 more
TL;DR: A novel protein purification system has been developed which enables purification of free recombinant proteins in a single chromatographic step using a modified protein splicing element from Saccharomyces cerevisiae in conjunction with a chitin-binding domain from Bacillus circulans as an affinity tag.
Journal ArticleDOI
HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription
Stephen E. Rundlett,Andrew A. Carmen,Ryuji Kobayashi,Sergei Bavykin,Bryan M. Turner,Michael Grunstein +5 more
TL;DR: The characterized yeast histone deacetylase complexes demonstrate that histone acetylation state has a role in regulating both heterochromatic silencing and regulated gene expression.
Journal ArticleDOI
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
TL;DR: A fourth member of this family of neuronal-specific Elav-like proteins that binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs is identified and cloned.
Journal ArticleDOI
MyD88, an adapter protein involved in interleukin-1 signaling.
Kimberly Burns,Fabio Martinon,Christoph Esslinger,Heike L. Pahl,Pascal Schneider,Jean-Luc Bodmer,Francesco Di Marco,Lars E. French,Jürg Tschopp +8 more
TL;DR: A role for MyD88 as an adapter in IL-1 signal transduction is supported; MyD 88 forms homodimers in vivo through DD-DD and Toll-Toll interactions.
References
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Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Journal ArticleDOI
Determination of protein: A modification of the lowry method that gives a linear photometric response
TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
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Supercoil sequencing: a fast and simple method for sequencing plasmid DNA
Ellson Y. Chen,Peter H. Seeburg +1 more
TL;DR: A method for obtaining sequence information directly from plasmid DNA is presented, and the advantages include speed, simplicity, avoidance of additional cloning steps into single-stranded phage M13 vectors, and hence applicability to sequencing large numbers of samples.
Journal ArticleDOI
Efficient isolation of genes by using antibody probes.
Richard A. Young,Ronald W. Davis +1 more
TL;DR: A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.