Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
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TLDR
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.About:
This article is published in Gene.The article was published on 1988-07-15. It has received 6003 citations till now. The article focuses on the topics: Fusion protein & Expression vector.read more
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Phosphorylation of Bim-EL by Erk1/2 on serine 69 promotes its degradation via the proteasome pathway and regulates its proapoptotic function.
Frederic Luciano,Arnaud Jacquel,Pascal Colosetti,Magali Herrant,Sebastien Cagnol,Gilles Pagès,Patrick Auberger +6 more
TL;DR: It is reported here that Bim-EL when phosphorylated by Erk1/2 is rapidly degraded via the proteasome pathway, and therefore represents a new and important mechanism of Bim regulation.
Journal ArticleDOI
Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae
TL;DR: It is shown that the APG1 gene, involved in the autophagic process in yeast, encodes a novel type of Ser/Thr protein kinase, suggesting that homologous molecular mechanisms, conserved from unicellular to multicellular organisms, are involved in dynamic membrane flow.
Journal ArticleDOI
Unsaturated fatty acids inhibit transcription of the sterol regulatory element-binding protein-1c (SREBP-1c) gene by antagonizing ligand-dependent activation of the LXR
Jiafu Ou,Hua Tu,Bei Shan,Alvin Luk,Russell A. DeBose-Boyd,Yuriy K. Bashmakov,Joseph L. Goldstein,Michael S. Brown +7 more
TL;DR: It is shown that uns saturated fatty acids lower SREBP-1c mRNA levels in part by antagonizing the actions of LXR, a potential mechanism that partially explains the long-known ability of dietary unsaturated fatty acids to decrease the synthesis and secretion of fatty acids and triglycerides in livers of humans and other animals.
Journal ArticleDOI
Bcr encodes a GTPase-activating protein for p21rac.
Dagmar Diekmann,Suzanne Brill,Michelle D. Garrett,Nicholas F. Totty,J. Justin Hsuan,Clinton Monfries,Christine Hall,Louis Lim,Alan Hall +8 more
TL;DR: It is shown that the carboxy-terminal domains of the bcr-encoded protein (Bcr) and of a Bcr-related protein, n-chimaerin, are both GAP proteins for the Ras-related GTP-binding protein, p21rac, which suggests that Bcr could be a target for regulation by Rac and has important new implications for the role of bcr translocations in leukaemia.
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Human cytomegalovirus IE1 and IE2 proteins block apoptosis.
Hua Zhu,Y Shen,Thomas Shenk +2 more
TL;DR: The results suggest a new physiological role for the IE1 and IE2 proteins in the human cytomegalovirus replication cycle.
References
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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Journal ArticleDOI
Determination of protein: A modification of the lowry method that gives a linear photometric response
TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
Journal ArticleDOI
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA
Ellson Y. Chen,Peter H. Seeburg +1 more
TL;DR: A method for obtaining sequence information directly from plasmid DNA is presented, and the advantages include speed, simplicity, avoidance of additional cloning steps into single-stranded phage M13 vectors, and hence applicability to sequencing large numbers of samples.
Journal ArticleDOI
Efficient isolation of genes by using antibody probes.
Richard A. Young,Ronald W. Davis +1 more
TL;DR: A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.