Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
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TLDR
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.About:
This article is published in Gene.The article was published on 1988-07-15. It has received 6003 citations till now. The article focuses on the topics: Fusion protein & Expression vector.read more
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Protein farnesyltransferase and geranylgeranyltransferase share a common α subunit
TL;DR: It is shown here that the α subunit of Mammalian farnesyltransferase is shared with another prenyl transferase that attaches 20 carbon geranylgeranyl to Ras-related proteins.
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Binding of general transcription factor TFIIB to an acidic activating region.
TL;DR: TFIIB expressed in and purified from Escherichia coli (recombinant TFIIB) binds directly to the potent acidic activating region of the herpes simplex virus-1 VP16 protein.
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hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II.
TL;DR: The cloning and characterization of a novel human TBP‐associated factor, hTAF(II)68, which contains a consensus RNA‐binding domain (RNP‐CS) and binds not only RNA, but also single stranded DNA, is reported.
Journal ArticleDOI
Heterogeneous nuclear ribonucleoprotein K is a transcription factor.
TL;DR: Data indicate that hnRNP K binds to a specific cis element, interacts with the RNA polymerase II transcription machinery, and stimulates transcription and thus has all of the properties of a transcription factor.
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Mislocalization of delta F508 CFTR in cystic fibrosis sweat gland.
TL;DR: The generated monoclonal antibodies to CFTR demonstrate that the biosynthetic arrest and intracellular retention of ΔF508 GFTR initially observed in vitro does occur in vivo and emphasizes the need to focus efforts on understanding the mislocalization.
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Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
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Determination of protein: A modification of the lowry method that gives a linear photometric response
TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
Journal ArticleDOI
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA
Ellson Y. Chen,Peter H. Seeburg +1 more
TL;DR: A method for obtaining sequence information directly from plasmid DNA is presented, and the advantages include speed, simplicity, avoidance of additional cloning steps into single-stranded phage M13 vectors, and hence applicability to sequencing large numbers of samples.
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Efficient isolation of genes by using antibody probes.
Richard A. Young,Ronald W. Davis +1 more
TL;DR: A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.