Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
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TLDR
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.About:
This article is published in Gene.The article was published on 1988-07-15. It has received 6003 citations till now. The article focuses on the topics: Fusion protein & Expression vector.read more
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Oligomerization and phosphorylation of the Ire1p kinase during intracellular signaling from the endoplasmic reticulum to the nucleus.
Caroline E. Shamu,Peter Walter +1 more
TL;DR: Molecular genetic and biochemical studies described here suggest that, as in the case of growth factor receptors of higher eukaryotic cells, Ire1p oligomerizes in response to the accumulation of unfolded proteins in the ER and is phosphorylated in trans by otherIre1p molecules as a result of oligomerization.
Journal ArticleDOI
LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
Yukiko Kabeya,Noboru Mizushima,Noboru Mizushima,Takashi Ueno,Akitsugu Yamamoto,Takayoshi Kirisako,Takayoshi Kirisako,Takeshi Noda,Takeshi Noda,Eiki Kominami,Yoshinori Ohsumi,Yoshinori Ohsumi,Tamotsu Yoshimori,Tamotsu Yoshimori +13 more
TL;DR: It is demonstrated that the rat microtubule‐associated protein 1 light chain 3 (LC3), a homologue of Apg8p essential for autophagy in yeast, is associated to the autophagosome membranes after processing.
Journal ArticleDOI
Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae
Mark S. Longtine,Amos Mckenzie,Douglas J. Demarini,Nirav Shah,Achim Wach,Arndt Brachat,Peter Philippsen,John R. Pringle +7 more
TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.
Journal ArticleDOI
The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors.
Anne J. Ridley,Alan Hall +1 more
TL;DR: Rho, a ras-related GTP-binding protein, rapidly stimulated stress fiber and focal adhesion formation when microinjected into serum-starved Swiss 3T3 cells, implying that rho is essential specifically for the coordinated assembly of focal adhesions and stress fibers induced by growth factors.
Journal ArticleDOI
Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes
Jun Liu,Jesse D. Farmer,Willam S. Lane,Jeffrey S. Friedman,Irving L. Weissman,Stuart L. Schreiber +5 more
TL;DR: The results suggest that calcineurin is involved in a common step associated with T cell receptor and IgE receptor signaling pathways and that cyclophilin and FKBP mediate the actions of CsA and Fk506 by forming drug-dependent complexes with and altering the activity of calcineURin-calmodulin.
References
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Supercoil sequencing: a fast method for sequencing plasmid DNA
Ellson Y. Chen,Peter H. Seeburg +1 more
Journal ArticleDOI
Maturation of the head of bacteriophage T4. II. Head-related, aberrant tau-particles.
TL;DR: Temperature shift-down experiments, using several different phage carrying temperature-sensitive mutations in gene 21 , indicate that the bulk of the τ-particles cannot be used for normal phage production and strongly suggest that cleavage of the head proteins is required for DNA packaging to occur.
Journal ArticleDOI
The tac promoter: a functional hybrid derived from the trp and lac promoters
TL;DR: Two hybrid promoters that are functional in Escherichia coli have been constructed and are useful for the controlled expression of foreign genes at high levels in E. coli.
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Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin
Keiichi Itakura,Tadaaki Hirose,Roberto Crea,Arthur D. Riggs,Herbert L. Heyneker,Francisco Bolívar,Herbert W. Boyer +6 more
TL;DR: This work has reported the first synthesis of a functional polypeptide product from a gene of chemically synthesized origin, including the sequence of amino acids corresponding to somatostatin, in vitro.
Journal ArticleDOI
The purification of eukaryotic polypeptides synthesized in Escherichia coli.
TL;DR: From the increasing number of reports of eukaryotic polypeptide synthesis in E. coli it is clear that the mode of expression affects not only the efficiency of production, but the nature of the polypePTide product itself.