Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
Reads0
Chats0
TLDR
Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.About:
This article is published in Gene.The article was published on 1988-07-15. It has received 6003 citations till now. The article focuses on the topics: Fusion protein & Expression vector.read more
Citations
More filters
Journal ArticleDOI
A novel divalent cation-binding site in the a domain of the β2 integrin CR3 (CD11b/CD18) is essential for ligand binding
M Michishita,V Videm,M A Arnaout +2 more
TL;DR: The findings identify an unsuspected and novel metal-binding site within the A domain of CR3 that is required for metal-dependent ligand binding and also identify a good target for designing drugs aimed at countering the inflammatory potential of this key receptor.
Journal ArticleDOI
The mouse type IV c-abl gene product is a nuclear protein, and activation of transforming ability is associated with cytoplasmic localization
TL;DR: The subcellular localization of the mouse type IV c-abl protein was determined by indirect immunofluorescence of nontransformed NIH 3T3 fibroblasts that overexpress the protein, revealing a nuclear localization signal similar to that of SV40 large T antigen.
Journal ArticleDOI
Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction.
TL;DR: It is shown that translocation requires two redundant nuclear localization sequences and that one sequence is in an intramolecular association with phosphorserines in a conserved motif located at the amino terminus of each NF-AT protein.
Journal ArticleDOI
Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth.
Arthur J Molendijk,Friedrich Bischoff,Chadalavada S V Rajendrakumar,Jiří Friml,Markus Braun,Simon Gilroy,Klaus Palme +6 more
TL;DR: A general role for some members of this family in polarized plant growth processes is reported and a general function of AtRop GTPases in tip growth and in polar diffuse growth is demonstrated.
Journal Article
Laminin-5 Is a Marker of Invading Cancer Cells in Some Human Carcinomas and Is Coexpressed with the Receptor for Urokinase Plasminogen Activator in Budding Cancer Cells in Colon Adenocarcinomas
TL;DR: The colocalization of laminin-5 and urokinase-type plasminogen activator receptor in a subset of cancer cells in colon cancer suggests that a controlled up-regulation of a number of gene products is a characteristic of budding colon cancer cells, and that these gene products serve functions crucial for the invasive phenotype of these cancer cells.
References
More filters
Journal ArticleDOI
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
Journal ArticleDOI
Determination of protein: A modification of the lowry method that gives a linear photometric response
TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
Journal ArticleDOI
Supercoil sequencing: a fast and simple method for sequencing plasmid DNA
Ellson Y. Chen,Peter H. Seeburg +1 more
TL;DR: A method for obtaining sequence information directly from plasmid DNA is presented, and the advantages include speed, simplicity, avoidance of additional cloning steps into single-stranded phage M13 vectors, and hence applicability to sequencing large numbers of samples.
Journal ArticleDOI
Efficient isolation of genes by using antibody probes.
Richard A. Young,Ronald W. Davis +1 more
TL;DR: A sensitive and general technique has been devised for the dual purposes of cloning genes by using antibodies as probes and isolating unknown proteins encoded by cloned DNA using an expression vector that permits insertion of foreign DNA into the beta-galactosidase structural gene lacZ and promotes synthesis of hybrid proteins.