Journal ArticleDOI
Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: I. Instrumentation, imaging and spectroscopy.
Jörg Maser,A. Osanna,Yuxin Wang,Chris Jacobsen,Janos Kirz,Stephen A. Spector,Stephen A. Spector,Barry Winn,D. Tennant +8 more
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TLDR
A cryo scanning transmission X‐ray microscope which uses soft X‐rays from the National Synchrotron Light Source is developed, capable of imaging frozen hydrated specimens with a thickness of up to 10 μm at temperatures of around 100 K, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 1010 Gray.Abstract:
We have developed a cryo scanning transmission X-ray microscope which uses soft X-rays from the National Synchrotron Light Source The system is capable of imaging frozen hydrated specimens with a thickness of up to 10 microm at temperatures of around 100 K We show images and spectra from frozen hydrated eukaryotic cells, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 1010 Gray This makes possible studies where multiple images of the same specimen area are needed, such as tomography (Wang et al (2000) Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: II Tomography J Microsc 197, 80-93) or spectroscopic analysisread more
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Interferometer‐controlled scanning transmission X‐ray microscopes at the Advanced Light Source
A. L. D. Kilcoyne,Tolek Tyliszczak,Tolek Tyliszczak,W. F. Steele,Sirine C. Fakra,P. Hitchcock,P. Hitchcock,K. Franck,Erik H. Anderson,Bruce Harteneck,Ed G. Rightor,G. E. Mitchell,Adam P. Hitchcock,L. Yang,Tony Warwick,Harald Ade +15 more
TL;DR: Two new soft X-ray scanning transmission microscopes located at the Advanced Light Source (ALS) have been designed, built and commissioned and interferometer control implemented in both microscopes allows the precise measurement of the transverse position of the zone plate relative to the sample.
Journal ArticleDOI
Biological imaging by soft x-ray diffraction microscopy
David A. Shapiro,Pierre Thibault,T. Beetz,T. Beetz,Veit Elser,Malcolm R. Howells,Chris Jacobsen,Chris Jacobsen,Janos Kirz,Janos Kirz,E. Lima,H. Miao,Aaron M. Neiman,David Sayre +13 more
TL;DR: This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.
Journal ArticleDOI
An assessment of the resolution limitation due to radiation-damage in X-ray diffraction microscopy
Malcolm R. Howells,T. Beetz,Henry N. Chapman,C. Cui,James M. Holton,James M. Holton,Chris Jacobsen,Chris Jacobsen,Janos Kirz,Janos Kirz,E. Lima,Stefano Marchesini,H. Miao,David Sayre,David A. Shapiro,John C. H. Spence,John C. H. Spence,D. Starodub +17 more
TL;DR: The conclusion of this study is that, based on the natural contrast between protein and water and "Rose-criterion" image quality, one should be able to image a frozen-hydrated biological sample using XDM at a resolution of about 10 nm.
Journal ArticleDOI
NEXAFS microscopy and resonant scattering: Composition and orientation probed in real and reciprocal space
Harald Ade,Adam P. Hitchcock +1 more
TL;DR: In this article, the authors provide an overview of the state-of-the-art synchrotron-based tools for soft X-ray characterization and discuss a number of applications to exemplify their unique aspects.
Journal ArticleDOI
Multiple Scattering Calculations of Bonding and X-ray Absorption Spectroscopy of Manganese Oxides
Benjamin Gilbert,B. H. Frazer,A. Belz,P. G. Conrad,Kenneth H. Nealson,Daniel Haskel,Jonathan Lang,George Srajer,G. De Stasio +8 more
TL;DR: In this paper, the authors present near edge X-ray absorption spectra of manganese oxides at the Mn L2,3, Mn K, and O K edges to investigate the relative sensitivity of the edges to bonding and structure.
References
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X-Ray Interactions: Photoabsorption, Scattering, Transmission, and Reflection at E = 50-30,000 eV, Z = 1-92
TL;DR: In this article, the atomic scattering factors for all angles of coherent scattering and at the higher photon energies are obtained from these tabulated forward-scattering values by adding a simple angle-dependent form-factor correction.
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Cryo-electron microscopy of vitrified specimens.
Jacques Dubochet,Marc Adrian,J.-J. Chang,Jean-Claude Homo,J. Lepault,Alasdair W. McDowall,Patrick Schultz +6 more
TL;DR: Water is the most abundant component of biological material, but it is systematically excluded from conventional electron microscopy, because water evaporates rapidly under the vacuum conditions of an electron microscope.
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Breaking the Diffraction Barrier: Optical Microscopy on a Nanometric Scale
TL;DR: A near-field probe has been developed that yields a resolution of ∼12 nm (∼λ/43) and signals ∼104- to 106-fold larger than those reported previously and image contrast is demonstrated to be highly polarization dependent.
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Spiegelsysteme streifenden Einfalls als abbildende Optiken für Röntgenstrahlen
TL;DR: In this article, the authors define Optiken zur Rontgenstrahlmikroskopie eignen sich Systeme von total-reflektierenden Spiegeln, die bei Hebung der spharischen Aberration fur einen Achsenpunkt zugleich die Abbesche Sinusbedingung bis zu Aperturen 0,05 befriedigend erfullen.
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Scanning interferometric apertureless microscopy: optical imaging at 10 angstrom resolution.
TL;DR: Interferometric near-field optical microscopy achieving a resolution of 10 angstroms is demonstrated, sensing the dipole-dipole coupling of two externally driven dipoles as their spacing is modulated.