Status update in the use of cell-penetrating peptides for the delivery of macromolecular therapeutics.
TLDR
Most important clinical developments have occurred with the CPP-drug conjugate approaches that target various protein–protein interactions, and these have been highlighted subsequently.Abstract:
In this review, recent developments and applications with cell-penetrating peptides (CPP) are discussed. CPPs are widely used tools for the delivery of various macromolecular therapeutics, such as ...read more
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Cell-penetrating peptides in protein mimicry and cancer therapeutics
TL;DR: In this article , a review of cell-penetrating peptides (CPPs) and their potential roles in cancer immunotherapy and diagnostics is presented, highlighting some of the most recent achievements in selective delivery of anticancer drugs to a range of tumor cells both in vitro and in vivo.
Journal ArticleDOI
Cell-penetrating peptides in protein mimicry and cancer therapeutics.
TL;DR: In this article, a review of cell-penetrating peptides (CPPs) and their potential roles in cancer immunotherapy and diagnostics is presented, highlighting some of the most recent achievements in selective delivery of anticancer drugs to a range of tumor cells both in vitro and in vivo.
Journal ArticleDOI
Bioconjugate Supramolecular Pd2+ Metallacages Penetrate the Blood Brain Barrier In Vitro and In Vivo.
Ben Woods,Rúben D M Silva,Claudia Schmidt,Darren Wragg,Marco Cavaco,Vera Neves,Vera F. C. Ferreira,Lurdes Gano,Tânia S. Morais,Filipa Mendes,João D. G. Correia,Angela Casini +11 more
TL;DR: In this paper, a new water-soluble homoleptic cage (CPepH3) tethered to a blood brain barrier (BBB)-translocating peptide was synthesized by a combination of solid phase peptide synthesis (SPPS) and self-assembly procedures.
Journal ArticleDOI
How Do Biomolecules Cross the Cell Membrane?
TL;DR: It is demonstrated that CPPs and bacterial protein toxins escape the endosome by the VBC mechanism in giant unilamellar vesicles as well as live mammalian cells and it is proposed that VBC may be the mechanism that drives the bacterial TAT and eukaryotic UPS systems.
Journal ArticleDOI
Cell-Penetrating Peptides and Transportan.
TL;DR: In the most recent 25-30 years, multiple novel mechanisms and applications of cell-penetrating peptides (CPP) have been demonstrated, leading to novel drug delivery systems as mentioned in this paper.
References
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Journal ArticleDOI
Cellular uptake of the tat protein from human immunodeficiency virus
Alan D. Frankel,Carl O. Pabo +1 more
TL;DR: Experiments using radioactive protein show that tat becomes localized to the nucleus after uptake and suggest that chloroquine protects tat from proteolytic degradation, raising the possibility that, under some conditions, tat might act as a viral growth factor to stimulate viral replication in latently infected cells or alter expression of cellular genes.
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Tumor imaging by means of proteolytic activation of cell-penetrating peptides.
TL;DR: In mice xenografted with human tumor cells secreting matrix metalloproteinases 2 and 9, ACPPs bearing a far-red-fluorescent cargo show in vivo contrast ratios of 2-3 and a 3.1-fold increase in standard uptake value for tumors relative to contralateral normal tissue or control peptides with scrambled linkers.
Journal ArticleDOI
Design of a peptide-based vector, PepFect6, for efficient delivery of siRNA in cell culture and systemically in vivo.
Samir El Andaloussi,Taavi Lehto,Imre Mäger,Katri Rosenthal-Aizman,Iulian I. Oprea,Oscar E. Simonson,Helena Sork,Kariem Ezzat,Dana Copolovici,Kaido Kurrikoff,Joana R Viola,Eman M. Zaghloul,Rannar Sillard,Henrik J. Johansson,Fatouma Said Hassane,Peter Guterstam,Julia Suhorutsenko,Pedro Moreno,Nikita Oskolkov,Jonas Hälldin,Ulf Tedebark,Andres Metspalu,Bernard Lebleu,Janne Lehtiö,C. I. Edvard Smith,Ülo Langel +25 more
TL;DR: A novel CPP, PepFect 6 (PF6), comprising the previously reported stearyl-TP10 peptide, having pH titratable trifluoromethylquinoline moieties covalently incorporated to facilitate endosomal release is presented, implying that the peptides, in addition to having utility for RNAi screens in vitro, displays therapeutic potential.
Journal ArticleDOI
Overcoming Endosomal Entrapment in Drug Delivery
Dehua Pei,Marina Yu. Buyanova +1 more
TL;DR: This Topical Review provides an overview of the recent progress in understanding and enhancing the endosomal escape process and the new opportunities opened up by these recent findings.
Journal ArticleDOI
Cell-penetrating peptides for the delivery of nucleic acids
TL;DR: This review will discuss recent advances of CPP-mediated delivery of nucleic acid-based cargo, concentrating on the delivery of plasmid DNA, splice-correcting ONs, and small-interfering RNAs.