Strigolactone Biosynthesis in Medicago truncatula and Rice Requires the Symbiotic GRAS-Type Transcription Factors NSP1 and NSP2
Wei Liu,Wei Liu,Wouter Kohlen,Alessandra Lillo,Rik Op den Camp,Sergey Ivanov,Marijke Hartog,Erik Limpens,Muhammad Jamil,Cezary Smaczniak,Kerstin Kaufmann,Wei-Cai Yang,Guido J. E. J. Hooiveld,Tatsiana Charnikhova,Harro J. Bouwmeester,Ton Bisseling,Ton Bisseling,René Geurts +17 more
TLDR
It is shown that NSP1 and NSP2 are indispensable for strigolactone (SL) biosynthesis in the legume Medicago truncatula and in rice, which implies that both proteins fulfill dual regulatory functions to control downstream targets after rhizobium-induced signaling as well as SL biosynthetic in nonsymbiotic conditions.Abstract:
Legume GRAS (GAI, RGA, SCR)-type transcription factors NODULATION SIGNALING PATHWAY1 (NSP1) and NSP2 are essential for rhizobium Nod factor-induced nodulation. Both proteins are considered to be Nod factor response factors regulating gene expression after symbiotic signaling. However, legume NSP1 and NSP2 can be functionally replaced by nonlegume orthologs, including rice (Oryza sativa) NSP1 and NSP2, indicating that both proteins are functionally conserved in higher plants. Here, we show that NSP1 and NSP2 are indispensable for strigolactone (SL) biosynthesis in the legume Medicago truncatula and in rice. Mutant nsp1 plants do not produce SLs, whereas in M. truncatula, NSP2 is essential for conversion of orobanchol into didehydro-orobanchol, which is the main SL produced by this species. The disturbed SL biosynthesis in nsp1 nsp2 mutant backgrounds correlates with reduced expression of DWARF27, a gene essential for SL biosynthesis. Rice and M. truncatula represent distinct phylogenetic lineages that split approximately 150 million years ago. Therefore, we conclude that regulation of SL biosynthesis by NSP1 and NSP2 is an ancestral function conserved in higher plants. NSP1 and NSP2 are single-copy genes in legumes, which implies that both proteins fulfill dual regulatory functions to control downstream targets after rhizobium-induced signaling as well as SL biosynthesis in nonsymbiotic conditions.read more
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Speak, friend, and enter: signalling systems that promote beneficial symbiotic associations in plants.
TL;DR: This Review describes the common signalling processes used by plants during mutualistic interactions with microorganisms as diverse as arbuscular mycorrhizal fungi and rhizobial bacteria.
Journal ArticleDOI
Strigolactones, a Novel Carotenoid-Derived Plant Hormone
TL;DR: This review focuses on SL biosynthesis, describes the hormonal and environmental factors that determine this process, and discusses SL transport and downstream signaling as well as the role of SLs in regulating plant development.
Journal ArticleDOI
A petunia ABC protein controls strigolactone-dependent symbiotic signalling and branching
Tobias Kretzschmar,Wouter Kohlen,Joelle Sasse,Lorenzo Borghi,Markus Schlegel,Julien B. Bachelier,Didier Reinhardt,Ralph Bours,Harro J. Bouwmeester,Enrico Martinoia +9 more
TL;DR: It is shown that the Petunia hybrida ABC transporter PDR1 has a key role in regulating the development of arbuscular mycorrhizae and axillary branches, by functioning as a cellular strigolactone exporter.
Journal ArticleDOI
Cell and developmental biology of arbuscular mycorrhiza symbiosis.
Caroline Gutjahr,Martin Parniske +1 more
TL;DR: An elegant study has unveiled a new and unexpected mechanism for specific protein localization to the periarbuscular membrane, which relies on the timing of gene expression to synchronize protein biosynthesis with a redirection of secretion.
Journal ArticleDOI
Strigolactone Signaling and Evolution.
TL;DR: This review focuses on the molecular mechanisms, core developmental roles, and evolutionary history of strigolactone signaling and proposes potential translational applications of strIGolactones research to agriculture.
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