Journal ArticleDOI
Structure of catabolite gene activator protein at 2.9 Å resolution suggests binding to left-handed B-DNA
David B. McKay,Thomas A. Steitz +1 more
TLDR
The 2.9 Å resolution crystal structure of Escherichia coli catabolite gene activator protein (CAP) completed with cyclic AMP reveals two distinct structural domains separated by a cleft, suggesting that the CAP conversion of right- to left-handed DNA in a closed supercoil, is what activates transcription by RNA polymerase.Abstract:
The 2.9 A resolution crystal structure of Escherichia coli catabolite gene activator protein (CAP) complexed with cyclic AMP reveals two distinct structural domains separated by a cleft. The smaller carboxy-terminal domain is presumed to bind DNA while the amino-terminal domain is seen to bind cyclic AMP. Model building studies suggest that CAP binds to left-handed B-type DNA, contracting its major groove via two alpha-helices. It is possible that the CAP conversion of right- to left-handed DNA in a closed supercoil, is what activates transcription by RNA polymerase.read more
Citations
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Isolation and characterization of the SPT2 gene, a negative regulator of Ty-controlled yeast gene expression.
TL;DR: The cloning and DNA sequence analysis of the wild-type SPT2 gene is reported and it is shown that the gene is capable of encoding a protein of 333 amino acids in length and that a dominant mutation of the SPT1 gene results from the generation of an ochre codon which is presumed to lead to a shortened SPT 2 gene product.
Journal ArticleDOI
Purification and properties of a transcriptional activator. The cII protein of phage lambda.
Y Ho,M Lewis,M Rosenberg +2 more
TL;DR: It is demonstrated that cII in solution is a tetrameric protein and that it undergoes specific processing at its NH2-terminal end and is characterized as to its molar extinction coefficient, molecular weight, amino acid composition, isoelectric point, alpha-helical content, and antigenic capability.
Journal ArticleDOI
Quality and efficiency of basic research in molecular biology: a bibliometric analysis of thirteen excellent research institutes
TL;DR: The number of scientific publications and the number of citations received during a five-year period (1980–1984) are counted and citations per publication and costs per citation are used as an indicator of quality and efficiency of research.
Journal ArticleDOI
Z-DNA in the rat somatostatin gene.
T E Hayes,J E Dixon +1 more
TL;DR: It is proposed that the parallel placement of alternating purine-pyrimidine or potential Z-DNA-forming sequences 5' to the somatostatin genes from two species is a result of structural, in contrast to sequence, conservation.
Journal ArticleDOI
Heme environmental structure of CooA is modulated by the target DNA binding. Evidence from resonance Raman spectroscopy and CO rebinding kinetics.
Takeshi Uchida,Haruto Ishikawa,Satoshi Takahashi,Koichiro Ishimori,Isao Morishima,Kei Ohkubo,Hiroshi Nakajima,Shigetoshi Aono +7 more
TL;DR: The structural alterations in the heme distal site in response to binding of the target DNA support the activation mechanism proposed for CooA, which is triggered by the movement of the he me distal ligand to modify the conformation of the DNA binding domain.
References
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Journal ArticleDOI
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.
TL;DR: The haemagglutinin glycoprotein of influenza virus is a trimer comprising two structurally distinct regions: a triple-stranded coiled-coil of α-helices extends 76 Å from the membrane and a globular region of antiparallel β-sheet is positioned on top of this stem.
Journal ArticleDOI
Molecular structure of a left-handed double helical DNA fragment at atomic resolution
Andrew H.-J. Wang,Gary J. Quigley,F. Kolpak,J.L. Crawford,J. H. Van Boom,G. A. Van Der Marel,Alexander Rich +6 more
TL;DR: The DNA fragment d(CpGpCpC pGp CpG pG) crystallises as a left-handed double helical molecule with Watson–Crick base pairs and an antiparallel organisation of the sugar phosphate chains.
Journal ArticleDOI
Optimised parameters for A-DNA and B-DNA
TL;DR: The molecular structures presented have the most probable values of bond-lengths, bond-angles and furanose ring conformations as defined by accurate X-ray crystallographic analyses of relevant monomers.
Journal ArticleDOI
Three-Dimensional Structure of Immunoglobulins
L M Amzel,Roberto J. Poljak +1 more
TL;DR: This chapter discusses a study analyzing the three-dimensional structure of immunoglobulins, in which the periodicity of the crystal was used to reduce the background noise and reveal the molecular outline.
Journal ArticleDOI
Tomato bushy stunt virus at 2.9 A resolution.
Stephen C. Harrison,Arthur J. Olson,Clarence E. Schutt,Clarence E. Schutt,Frank Winkler,Frank Winkler,Gérard Bricogne +6 more
TL;DR: The polypeptide chain of a TBSV subunit folds into two domains, connected by a hinge, and a flexibly-linked N-terminal arm, and RNA is also not uniquely fixed to sites on the major domains.
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