Journal ArticleDOI
Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium
J. Anjani Kumari,Tapobrata Panda +1 more
TLDR
The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml;Mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzyme, 2 h; temperature of protoplasts, 30°C; phosphate buffer, 25 m m , pH 6.5; KCl as osmotic stabilizer, 0.7 mAbout:
This article is published in Enzyme and Microbial Technology.The article was published on 1992-03-01. It has received 43 citations till now. The article focuses on the topics: Trichoderma longibrachiatum & Mycelium.read more
Citations
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Journal ArticleDOI
The role of trichoderma harzianum protease in the biocontrol of botrytis cinerea
Yigal Elad,A. Kapat +1 more
TL;DR: Protease inhibitors, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E64), antipain hydrochloride, and a mixture of inhibitors, but not pepstatin A, fully or partially nullified the biocontrol effect of T39.
Journal ArticleDOI
Low molecular weight chitosan prepared with the aid of cellulase, lysozyme and chitinase: Characterisation and antibacterial activity
TL;DR: LMWC enzyme-directed properties suggest chitinase is more predictable and flexible to produce the specified MV of LMWC, and E. coli strains showed much higher susceptibility to LMWCs than Staphylococcus aureus strains did and both species showed intra-species sensitivity diversity toward LMWc.
Journal ArticleDOI
Regulation and cloning of microbial chitinase genes.
P. A. Felse,Tapobrata Panda +1 more
TL;DR: A brief survey of recent progress in the regulation and cloning of microbial chitinase genes is given and emphasis is placed on the post-translational modification and localization of the recombinant protein in the host.
Journal ArticleDOI
Isolation of Trichoderma and Evaluation of their Antagonistic Potential against Alternaria porri
TL;DR: This study proved that T. harzianum Th-3013 as a biocontrol agent showed significant reduction in onion purple blotch disease compared with the tested fungicide.
Journal ArticleDOI
Preparation of antibacterial chito‐oligosaccharide by altering the degree of deacetylation of β‐chitosan in a Trichoderma harzianum chitinase‐hydrolysing process
TL;DR: To produce various sizes of COS for versatile biological functions, as seen in this study to inhibit various types of bacteria, is made possible in this established process.
References
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Journal ArticleDOI
Trichoderma protoplast fusion: a tool for improving biocontrol agents
TL;DR: The antagonistic abilities of the prototrophic strains were found to vary with each pathogenic fungus, and the protOTrophic strain A2 overgrew all the pathogenic fungi more rapidly than the parental strains.
Journal ArticleDOI
Fine structure of protoplasts of Aspergillus nidulans.
R. K. Gibson,J. F. Peberdy +1 more
TL;DR: Observations on the ultrastructure of Aspergillus nidulans protoplasts obtained by treatment of mycelium with Streptomyces RA lytic enzyme show that they were free of wall material.
Journal ArticleDOI
A simple method for protoplast formation and improvement of protoplast regeneration from various fungi using an enzyme from Trichoderma harzianum
TL;DR: An enzyme from Trichoderma harzianum dissolved the cell walls of a wide range of filamentous fungi and so could be used to make protoplasts and regenerated at about 1.8 times the rate of those produced by the original enzyme.
Journal ArticleDOI
Limited vegetative compatibility following intra- and interspecific protoplast fusion inTrichoderma
TL;DR: Limited compatibility may reduce the likelihood of parasexual recombination but does not preclude the possibility of genetic manipulation of Trichoderma strains by protoplast fusion.
Journal ArticleDOI
Interspecific protoplast fusion and complementation in aspergilli
L. Ferenczy,M. Szegedi,F. Kevei +2 more
TL;DR: It is concluded that the nutritional complementation in Aspergillus nidulans mutants may be due to interspecific aneuploidy.