Journal ArticleDOI
Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium
J. Anjani Kumari,Tapobrata Panda +1 more
TLDR
The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml;Mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzyme, 2 h; temperature of protoplasts, 30°C; phosphate buffer, 25 m m , pH 6.5; KCl as osmotic stabilizer, 0.7 mAbout:
This article is published in Enzyme and Microbial Technology.The article was published on 1992-03-01. It has received 43 citations till now. The article focuses on the topics: Trichoderma longibrachiatum & Mycelium.read more
Citations
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Studies on a N-acetyl-β-D-glucosaminidase produced by Fusarium oxysporum F3 grown in solid-state fermentation
Konstantinos Gkargkas,Diomi Mamma,Georgi Nedev,Evangelos Topakas,Paul Christakopoulos,Dimitris Kekos,Basil J. Macris +6 more
TL;DR: In this paper, two isozymes of N -acetyl-β-d-glucosaminidase were isolated from the culture filtrate of Fusarium oxysporum F3 and were subjected to ammonium sulphate fractionation followed by anion exchange, gel filtration, hydrophobic interaction and cation exchange chromatography.
Journal ArticleDOI
pH and thermal stability studies of chitinase from Trichoderma harzianum: A thermodynamic consideration
A. Kapat,Tapobrata Panda +1 more
TL;DR: In this paper, the combined effect of pH and temperature on chitinase was investigated using response surface methodology and a central composite design for two variables was employed, which was found out to be 5.4 and 24°C respectively.
Journal ArticleDOI
Statistical analysis on some critical parameters affecting the formation of protoplasts from the mycelium of Penicillium griseofulvum
TL;DR: The critical parameters, such as slant age, mycelium age and contact time of mycelial with lytic enzymes which affects the formation of protoplast from the Penicillium griseofulvum mycelia were studied using a novel technique called response surface methodology.
Journal ArticleDOI
Fermentation Conditions and Properties of a Chitosanase from Acinetobacter sp. C-17
Xu-Fen Zhu,Xiao-Yun Wu,Yun Dai +2 more
TL;DR: A species of bacterium with high chitosanase activity was isolated from soil samples in Haiyan City, China, and identified as an Acinetobacter species, named AcinetOBacter sp.
Journal ArticleDOI
Extractive fermentation for improved production of endoglucanase by an intergeneric fusant of Trichoderma reesei/Saccharomyces cerevisiae using aqueous two-phase system.
J Sinha,P.K Dey,Tapobrata Panda +2 more
TL;DR: Endoglucanase production is higher in medium containing polyethylene glycol (PEG) 6000 than in medium without PEG 6000, and the lower overall enzyme production by T. reesei in the two-phase system might be due to limitation in oxygen transfer to the dispersed phase where the enzyme is produced.
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
Fungal protoplasts: isolation, reversion, and fusion
TL;DR: The protoplast Fusion and the Hybridization of Fungal Species and Transformation in Fungi by Using Protoplasts by using Enzymatic and Nonenzymatic methods are studied.
Journal ArticleDOI
Induced fusion of fungal protoplasts following treatment with polyethylene glycol.
Jozef Anné,John F. Peberdy +1 more
TL;DR: Heterokaryon formation, at high frequency, after induced intraspecific fusion between protoplasts of auxotrophs of Penicillium chryosogiwim, P .
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A comparison of methods for estimating the viable count of a suspension of tumour cells.
TL;DR: All four in vitro methods of estimating the percentage viability of a cell suspension gave similar results with a recently harvested cell suspension but three of them grossly overestimated the proportion of viable cells in a suspension which had been stored at 4 °C for 11 days.