Journal ArticleDOI
Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium
J. Anjani Kumari,Tapobrata Panda +1 more
Reads0
Chats0
TLDR
The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml;Mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzyme, 2 h; temperature of protoplasts, 30°C; phosphate buffer, 25 m m , pH 6.5; KCl as osmotic stabilizer, 0.7 mAbout:
This article is published in Enzyme and Microbial Technology.The article was published on 1992-03-01. It has received 43 citations till now. The article focuses on the topics: Trichoderma longibrachiatum & Mycelium.read more
Citations
More filters
Journal ArticleDOI
The role of trichoderma harzianum protease in the biocontrol of botrytis cinerea
Yigal Elad,A. Kapat +1 more
TL;DR: Protease inhibitors, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E64), antipain hydrochloride, and a mixture of inhibitors, but not pepstatin A, fully or partially nullified the biocontrol effect of T39.
Journal ArticleDOI
Low molecular weight chitosan prepared with the aid of cellulase, lysozyme and chitinase: Characterisation and antibacterial activity
TL;DR: LMWC enzyme-directed properties suggest chitinase is more predictable and flexible to produce the specified MV of LMWC, and E. coli strains showed much higher susceptibility to LMWCs than Staphylococcus aureus strains did and both species showed intra-species sensitivity diversity toward LMWc.
Journal ArticleDOI
Regulation and cloning of microbial chitinase genes.
P. A. Felse,Tapobrata Panda +1 more
TL;DR: A brief survey of recent progress in the regulation and cloning of microbial chitinase genes is given and emphasis is placed on the post-translational modification and localization of the recombinant protein in the host.
Journal ArticleDOI
Isolation of Trichoderma and Evaluation of their Antagonistic Potential against Alternaria porri
TL;DR: This study proved that T. harzianum Th-3013 as a biocontrol agent showed significant reduction in onion purple blotch disease compared with the tested fungicide.
Journal ArticleDOI
Preparation of antibacterial chito‐oligosaccharide by altering the degree of deacetylation of β‐chitosan in a Trichoderma harzianum chitinase‐hydrolysing process
TL;DR: To produce various sizes of COS for versatile biological functions, as seen in this study to inhibit various types of bacteria, is made possible in this established process.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
Fungal protoplasts: isolation, reversion, and fusion
TL;DR: The protoplast Fusion and the Hybridization of Fungal Species and Transformation in Fungi by Using Protoplasts by using Enzymatic and Nonenzymatic methods are studied.
Journal ArticleDOI
Induced fusion of fungal protoplasts following treatment with polyethylene glycol.
Jozef Anné,John F. Peberdy +1 more
TL;DR: Heterokaryon formation, at high frequency, after induced intraspecific fusion between protoplasts of auxotrophs of Penicillium chryosogiwim, P .
Journal ArticleDOI
A comparison of methods for estimating the viable count of a suspension of tumour cells.
TL;DR: All four in vitro methods of estimating the percentage viability of a cell suspension gave similar results with a recently harvested cell suspension but three of them grossly overestimated the proportion of viable cells in a suspension which had been stored at 4 °C for 11 days.