Journal ArticleDOI
Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium
J. Anjani Kumari,Tapobrata Panda +1 more
TLDR
The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml;Mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzyme, 2 h; temperature of protoplasts, 30°C; phosphate buffer, 25 m m , pH 6.5; KCl as osmotic stabilizer, 0.7 mAbout:
This article is published in Enzyme and Microbial Technology.The article was published on 1992-03-01. It has received 43 citations till now. The article focuses on the topics: Trichoderma longibrachiatum & Mycelium.read more
Citations
More filters
Journal ArticleDOI
The role of trichoderma harzianum protease in the biocontrol of botrytis cinerea
Yigal Elad,A. Kapat +1 more
TL;DR: Protease inhibitors, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane (E64), antipain hydrochloride, and a mixture of inhibitors, but not pepstatin A, fully or partially nullified the biocontrol effect of T39.
Journal ArticleDOI
Low molecular weight chitosan prepared with the aid of cellulase, lysozyme and chitinase: Characterisation and antibacterial activity
TL;DR: LMWC enzyme-directed properties suggest chitinase is more predictable and flexible to produce the specified MV of LMWC, and E. coli strains showed much higher susceptibility to LMWCs than Staphylococcus aureus strains did and both species showed intra-species sensitivity diversity toward LMWc.
Journal ArticleDOI
Regulation and cloning of microbial chitinase genes.
P. A. Felse,Tapobrata Panda +1 more
TL;DR: A brief survey of recent progress in the regulation and cloning of microbial chitinase genes is given and emphasis is placed on the post-translational modification and localization of the recombinant protein in the host.
Journal ArticleDOI
Isolation of Trichoderma and Evaluation of their Antagonistic Potential against Alternaria porri
TL;DR: This study proved that T. harzianum Th-3013 as a biocontrol agent showed significant reduction in onion purple blotch disease compared with the tested fungicide.
Journal ArticleDOI
Preparation of antibacterial chito‐oligosaccharide by altering the degree of deacetylation of β‐chitosan in a Trichoderma harzianum chitinase‐hydrolysing process
TL;DR: To produce various sizes of COS for versatile biological functions, as seen in this study to inhibit various types of bacteria, is made possible in this established process.
References
More filters
Journal ArticleDOI
Induced segregation in interspecific hybrids of Aspergillus nidulans and Aspergillus rugulosus obtained by protoplast fusion.
Ferenc Kevei,John F. Peberdy +1 more
TL;DR: The findings from these experiments suggest that a high degree of chromosomal homology may exist between Aspergillus nidulans and As pergillus rugulosus, and that the two parents are closely related taxonomically.
Journal ArticleDOI
Sphaeroplast formation and regeneration in Trichoderma reesei
TL;DR: Sphaeroplasts from several genetically marked strains of Trichoderma reesei were readily prepared through enzymatic hydrolysis of mycelial suspensions utilizing Driselase, a commercially available lytic enzyme preparation, resulting in increased sphaeroplast yields from young as well as older mycelia.
Journal ArticleDOI
Interspecific protoplast fusion of Trichoderma
TL;DR: Protoplasts obtained from immature conidia of double auxotrophic mutant strains of Trichoderma reesei were fused with those of T. viride or T. koningii in the presence of polyethyleneglycol 6000, and the conidial DNA content of the knob clones was almost the same as that of the parents, independent of their phenotypes.
Journal ArticleDOI
Intraspecific hybridization of Trichoderma reesei QM 9414 by protoplast fusion using colour mutants
TL;DR: The production of well defined white and brown sectors on agar plates demonstrated that the fusants were heterokaryons and suggested the existence of a parasexual cycle in this species of Trichoderma.
Journal ArticleDOI
Production of protoplasts from the fungi Curvularia inaequalis and Trichoderma reesei
TL;DR: Protoplast formation in Curvularia inaequalis was achieved using non-commercial and commercial snail gut enzymes or Trichoderma harzianum enzymes and regeneration was succesfully carried out in semisolid agar.