Journal ArticleDOI
Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium
J. Anjani Kumari,Tapobrata Panda +1 more
TLDR
The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml;Mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzyme, 2 h; temperature of protoplasts, 30°C; phosphate buffer, 25 m m , pH 6.5; KCl as osmotic stabilizer, 0.7 mAbout:
This article is published in Enzyme and Microbial Technology.The article was published on 1992-03-01. It has received 43 citations till now. The article focuses on the topics: Trichoderma longibrachiatum & Mycelium.read more
Citations
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BookDOI
Biotechnological approaches in biocontrol of plant pathogens.
TL;DR: Host Parasite Specificity and Pathogenesis R.R. Tilak, K.G. Mukerji, and K.V. Srivastava.
Journal ArticleDOI
Submerged culture production of chitinase by Trichoderma harzianum in stirred tank bioreactors - The influence of agitator speed
P. Arthur Felse,Tapobrata Panda +1 more
TL;DR: Higher agitator speed appears to reduce chitinase production, Probably, mass transfer limitation was predominant in the fermentation process at lower agitATOR speed.
Journal ArticleDOI
Self-directing optimization of parameters for extracellular chitinase production by Trichoderma harzianum in batch mode
P. Arthur Felse,Tapobrata Panda +1 more
TL;DR: The self-directing optimization or the rotating simplex method of optimization was employed to determine the best suitable combination of parameters, pH (controlled), aeration rate and agitation rate for maximal production of chitinase by Trichoderma harzianum in batch culture.
Journal ArticleDOI
In vitro antagonism of Thielaviopsis paradoxa by Trichoderma longibrachiatum.
Vladimir Sánchez,Oscar Rebolledo,Rosa M. Picaso,Elizabeth Cárdenas,Jesús Córdova,Orfil González,Gary J. Samuels +6 more
TL;DR: Results demonstrate that the modes of action of T. longibrachiatum involved against Th.
Journal ArticleDOI
Biosynthesis of pathogenicity hydrolytic enzymes by Botrytis cinerea during infection of bean leaves and in vitro
TL;DR: Significant amounts of all hydrolytic enzymes studied in the early hours and monitored during 6 d of incubation were detected, supporting the possibility of a major role for the enzymes produced by B. cinerea in the development of disease.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
Fungal protoplasts: isolation, reversion, and fusion
TL;DR: The protoplast Fusion and the Hybridization of Fungal Species and Transformation in Fungi by Using Protoplasts by using Enzymatic and Nonenzymatic methods are studied.
Journal ArticleDOI
Induced fusion of fungal protoplasts following treatment with polyethylene glycol.
Jozef Anné,John F. Peberdy +1 more
TL;DR: Heterokaryon formation, at high frequency, after induced intraspecific fusion between protoplasts of auxotrophs of Penicillium chryosogiwim, P .
Journal ArticleDOI
A comparison of methods for estimating the viable count of a suspension of tumour cells.
TL;DR: All four in vitro methods of estimating the percentage viability of a cell suspension gave similar results with a recently harvested cell suspension but three of them grossly overestimated the proportion of viable cells in a suspension which had been stored at 4 °C for 11 days.