Journal ArticleDOI
Switching from repression to activation: microRNAs can up-regulate translation.
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TLDR
It is proposed that translation regulation by microRNPs oscillates between repression and activation during the cell cycle, and two well-studied microRNAs—Let-7 and the synthetic microRNA miRcxcr4—likewise induce translation up-regulation of target mRNAs on cell cycle arrest.Abstract:
AU-rich elements (AREs) and microRNA target sites are conserved sequences in messenger RNA (mRNA) 3' untranslated regions (3'UTRs) that control gene expression posttranscriptionally. Upon cell cycle arrest, the ARE in tumor necrosis factor-alpha (TNFalpha) mRNA is transformed into a translation activation signal, recruiting Argonaute (AGO) and fragile X mental retardation-related protein 1 (FXR1), factors associated with micro-ribonucleoproteins (microRNPs). We show that human microRNA miR369-3 directs association of these proteins with the AREs to activate translation. Furthermore, we document that two well-studied microRNAs-Let-7 and the synthetic microRNA miRcxcr4-likewise induce translation up-regulation of target mRNAs on cell cycle arrest, yet they repress translation in proliferating cells. Thus, activation is a common function of microRNPs on cell cycle arrest. We propose that translation regulation by microRNPs oscillates between repression and activation during the cell cycle.read more
Citations
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Journal ArticleDOI
The RNA-binding protein bicaudal C regulates polycystin 2 in the kidney by antagonizing miR-17 activity.
Uyen Tran,Lise Zakin,Axel Schweickert,Raman Agrawal,Remziye Döger,Martin Blum,E. M. De Robertis,Oliver Wessely +7 more
TL;DR: Data is provided that Bicaudal C functions by modulating the expression of polycystin 2 (Pkd2), a member of the transient receptor potential (TRP) superfamily, and it is proposed that the kidney phenotype in Bicc1−/− mutant mice is caused by dysregulation of a microRNA-based translational control mechanism.
Journal ArticleDOI
MicroRNAs (miRNAs) and Long Non-Coding RNAs (lncRNAs) as New Tools for Cancer Therapy: First Steps from Bench to Bedside.
Margherita Ratti,Andrea Lampis,Michele Ghidini,Massimiliano Salati,Milko Mirchev,Nicola Valeri,Nicola Valeri,Jens C. Hahne +7 more
TL;DR: The aim of this review is to summarise the latest knowledge of the use of miRNAs and lncRNAs as therapeutic tools for cancer treatment.
Journal ArticleDOI
5′–3′-UTR interactions regulate p53 mRNA translation and provide a target for modulating p53 induction after DNA damage
Jing Chen,Michael B. Kastan +1 more
TL;DR: Short, single-strand oligonucleotides that target this 5'-3'-UTR base-pairing region blunt the binding of RPL26 to p53 mRNA in cells and reduce p53 induction and p53-mediated cell death after several different types of DNA damage and cellular stress.
Journal ArticleDOI
Turnover of AU-rich-containing mRNAs during stress: a matter of survival.
TL;DR: How AUBPs regulate their target mRNAs in response to stress, along with the involvement of cytoplasmic granules in this process is discussed.
Journal ArticleDOI
MicroRNA-223 regulates FOXO1 expression and cell proliferation
Lihui Wu,Huihui Li,Cheng You Jia,Wei Cheng,Mei Yu,Min Peng,Yuanqing Zhu,Qianlei Zhao,Yi Wei Dong,Kang Shao,Anle Wu,Xing Zhong Wu +11 more
TL;DR: Interestingly, the expression levels of FOXO1 mRNA and protein, and phosphorylation levels became significantly lower than those of their control, and Proliferation of the cells was greatly inhibited when miR‐223 was over‐expressed.
References
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疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Control of translation and mRNA degradation by miRNAs and siRNAs
TL;DR: Possible mechanisms by which miRNAs control translation and mRNA degradation are discussed, an emerging theme is that mi RNAs, and siRNAs to some extent, target m RNAs to the general eukaryotic machinery for mRNA degradation and translation control.
Journal ArticleDOI
AU-rich elements: characterization and importance in mRNA degradation.
Chyi-Ying A. Chen,Ann-Bin Shyu +1 more
TL;DR: Observations suggest that Adenylate/uridylate-rich elements play a critical role in the regulation of gene expression during cell growth and differentiation and in the immune response.
Journal ArticleDOI
Specificity of microRNA target selection in translational repression
John G. Doench,Phillip A. Sharp +1 more
TL;DR: The ability of an miRNA to translationally repress a target mRNA is largely dictated by the free energy of binding of the first eight nucleotides in the 5' region of the miRNA, however, G:U wobble base-pairing in this region interferes with activity beyond that predicted on the basis of thermodynamic stability.
Journal ArticleDOI
From the U. S. A.
TL;DR: In this article, the existence of a monotone interpolating function with the same index set was shown to be necessary and sufficient for continuous interpolating functional if the index set I is finite, but not sufficient if I is infinite.
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