Journal ArticleDOI
Switching from repression to activation: microRNAs can up-regulate translation.
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TLDR
It is proposed that translation regulation by microRNPs oscillates between repression and activation during the cell cycle, and two well-studied microRNAs—Let-7 and the synthetic microRNA miRcxcr4—likewise induce translation up-regulation of target mRNAs on cell cycle arrest.Abstract:
AU-rich elements (AREs) and microRNA target sites are conserved sequences in messenger RNA (mRNA) 3' untranslated regions (3'UTRs) that control gene expression posttranscriptionally. Upon cell cycle arrest, the ARE in tumor necrosis factor-alpha (TNFalpha) mRNA is transformed into a translation activation signal, recruiting Argonaute (AGO) and fragile X mental retardation-related protein 1 (FXR1), factors associated with micro-ribonucleoproteins (microRNPs). We show that human microRNA miR369-3 directs association of these proteins with the AREs to activate translation. Furthermore, we document that two well-studied microRNAs-Let-7 and the synthetic microRNA miRcxcr4-likewise induce translation up-regulation of target mRNAs on cell cycle arrest, yet they repress translation in proliferating cells. Thus, activation is a common function of microRNPs on cell cycle arrest. We propose that translation regulation by microRNPs oscillates between repression and activation during the cell cycle.read more
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Journal ArticleDOI
Dicer1 Is Essential for Female Fertility and Normal Development of the Female Reproductive System
TL;DR: These studies implicate Dicer1/miRNA mediated posttranscriptional gene regulation in reproductive somatic tissues as critical for the normal development and function of these tissues and for female fertility.
Journal ArticleDOI
Estradiol Suppresses NF-κB Activation through Coordinated Regulation of let-7a and miR-125b in Primary Human Macrophages
TL;DR: It is demonstrated that pretreatment of human macrophages with estradiol attenuates LPS-induced TNF-α expression through the suppression of NF-κB activation, the first to demonstrate that estradio represses NF-kkB activation through the induction of κB-Ras2, a key inhibitor of NF -κB signaling.
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MicroRNAs: new players in IBD
Rahul Kalla,Nicholas T. Ventham,Nicholas A. Kennedy,Juan F. Quintana,Elaine R. Nimmo,Amy H. Buck,Jack Satsangi +6 more
TL;DR: The role of miRNAs in the development and regulation of the innate and adaptive immune system is discussed, with a particular focus on mechanisms pertinent to IBD and the potential translational applications.
Journal ArticleDOI
Normalization strategies for microRNA profiling experiments: a ‘normal’ way to a hidden layer of complexity?
TL;DR: A critical overview of commonly used and newly developed normalization methods for miRNA RT-qPCR, miRNA hybridization microarray, and small RNA-seq datasets is provided, with emphasis on the complexity, the importance and the potential for further optimization of normalization techniques for mi RNA profiling datasets.
Journal ArticleDOI
Hormonal regulation of microrna expression in periovulatory mouse mural granulosa cells
TL;DR: The following studies are the first to describe the extent of miRNA expression within ovarian granulosa cells and theFirst to demonstrate that LH/hCG regulates the expression of select miRNAs, which affect posttranscriptional gene regulation within these cells.
References
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疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Control of translation and mRNA degradation by miRNAs and siRNAs
TL;DR: Possible mechanisms by which miRNAs control translation and mRNA degradation are discussed, an emerging theme is that mi RNAs, and siRNAs to some extent, target m RNAs to the general eukaryotic machinery for mRNA degradation and translation control.
Journal ArticleDOI
AU-rich elements: characterization and importance in mRNA degradation.
Chyi-Ying A. Chen,Ann-Bin Shyu +1 more
TL;DR: Observations suggest that Adenylate/uridylate-rich elements play a critical role in the regulation of gene expression during cell growth and differentiation and in the immune response.
Journal ArticleDOI
Specificity of microRNA target selection in translational repression
John G. Doench,Phillip A. Sharp +1 more
TL;DR: The ability of an miRNA to translationally repress a target mRNA is largely dictated by the free energy of binding of the first eight nucleotides in the 5' region of the miRNA, however, G:U wobble base-pairing in this region interferes with activity beyond that predicted on the basis of thermodynamic stability.
Journal ArticleDOI
From the U. S. A.
TL;DR: In this article, the existence of a monotone interpolating function with the same index set was shown to be necessary and sufficient for continuous interpolating functional if the index set I is finite, but not sufficient if I is infinite.
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