Journal ArticleDOI
Switching from repression to activation: microRNAs can up-regulate translation.
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TLDR
It is proposed that translation regulation by microRNPs oscillates between repression and activation during the cell cycle, and two well-studied microRNAs—Let-7 and the synthetic microRNA miRcxcr4—likewise induce translation up-regulation of target mRNAs on cell cycle arrest.Abstract:
AU-rich elements (AREs) and microRNA target sites are conserved sequences in messenger RNA (mRNA) 3' untranslated regions (3'UTRs) that control gene expression posttranscriptionally. Upon cell cycle arrest, the ARE in tumor necrosis factor-alpha (TNFalpha) mRNA is transformed into a translation activation signal, recruiting Argonaute (AGO) and fragile X mental retardation-related protein 1 (FXR1), factors associated with micro-ribonucleoproteins (microRNPs). We show that human microRNA miR369-3 directs association of these proteins with the AREs to activate translation. Furthermore, we document that two well-studied microRNAs-Let-7 and the synthetic microRNA miRcxcr4-likewise induce translation up-regulation of target mRNAs on cell cycle arrest, yet they repress translation in proliferating cells. Thus, activation is a common function of microRNPs on cell cycle arrest. We propose that translation regulation by microRNPs oscillates between repression and activation during the cell cycle.read more
Citations
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Journal ArticleDOI
Regulation of eukaryotic gene expression by the untranslated gene regions and other non-coding elements
TL;DR: Recent insights into the regulatory roles of the untranslated gene regions and non-coding RNAs in the control of complex gene expression are discussed, as well as the implications of this in terms of organism complexity and evolution.
Journal ArticleDOI
Progress in microRNA delivery
TL;DR: The recent advances in the mechanisms and strategies of nonviral miRNA delivery systems are reviewed and a perspective on the future of miRNA-based therapeutics is provided.
Journal ArticleDOI
MicroRNA-21 Promotes Fibrosis of the Kidney by Silencing Metabolic Pathways
B. Nelson Chau,Cuiyan Xin,Jochen C. Hartner,Shuyu Ren,Ana P. Castano,Geoffrey Linn,Jian Li,Phong Tran,Vivek Kaimal,Xinqiang Huang,Aaron N. Chang,Shenyang Li,Aarti Kalra,Monica Grafals,Monica Grafals,Didier Portilla,Deidre Mackenna,Stuart H. Orkin,Jeremy S. Duffield +18 more
TL;DR: The finding that miR-21 is a major player in kidney fibrosis suggests that drugs that inhibit miR -21, like the complementary oligonucleotides used in this study, might prove to be useful therapies in humans.
Journal ArticleDOI
MicroRNA Regulatory Networks in Cardiovascular Development
Ning Liu,Eric N. Olson +1 more
TL;DR: The roles of miRNAs as regulators of cardiac form and function, unresolved questions in the field, and issues for the future are discussed.
Journal ArticleDOI
Dicer-dependent endothelial microRNAs are necessary for postnatal angiogenesis.
Yajaira Suárez,Carlos Fernández-Hernando,Jun Yu,Scott A. Gerber,Kenneth D. Harrison,Jordan S. Pober,M. Luisa Iruela-Arispe,Matthias Merkenschlager,William C. Sessa +8 more
TL;DR: Reduction of endothelial miRNAs by cell-specific inactivation of Dicer reduces postnatal angiogenic response to a variety of stimuli, including exogenous VEGF, tumors, limb ischemia, and wound healing.
References
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疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Control of translation and mRNA degradation by miRNAs and siRNAs
TL;DR: Possible mechanisms by which miRNAs control translation and mRNA degradation are discussed, an emerging theme is that mi RNAs, and siRNAs to some extent, target m RNAs to the general eukaryotic machinery for mRNA degradation and translation control.
Journal ArticleDOI
AU-rich elements: characterization and importance in mRNA degradation.
Chyi-Ying A. Chen,Ann-Bin Shyu +1 more
TL;DR: Observations suggest that Adenylate/uridylate-rich elements play a critical role in the regulation of gene expression during cell growth and differentiation and in the immune response.
Journal ArticleDOI
Specificity of microRNA target selection in translational repression
John G. Doench,Phillip A. Sharp +1 more
TL;DR: The ability of an miRNA to translationally repress a target mRNA is largely dictated by the free energy of binding of the first eight nucleotides in the 5' region of the miRNA, however, G:U wobble base-pairing in this region interferes with activity beyond that predicted on the basis of thermodynamic stability.
Journal ArticleDOI
From the U. S. A.
TL;DR: In this article, the existence of a monotone interpolating function with the same index set was shown to be necessary and sufficient for continuous interpolating functional if the index set I is finite, but not sufficient if I is infinite.
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