Journal ArticleDOI
Switching from repression to activation: microRNAs can up-regulate translation.
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TLDR
It is proposed that translation regulation by microRNPs oscillates between repression and activation during the cell cycle, and two well-studied microRNAs—Let-7 and the synthetic microRNA miRcxcr4—likewise induce translation up-regulation of target mRNAs on cell cycle arrest.Abstract:
AU-rich elements (AREs) and microRNA target sites are conserved sequences in messenger RNA (mRNA) 3' untranslated regions (3'UTRs) that control gene expression posttranscriptionally. Upon cell cycle arrest, the ARE in tumor necrosis factor-alpha (TNFalpha) mRNA is transformed into a translation activation signal, recruiting Argonaute (AGO) and fragile X mental retardation-related protein 1 (FXR1), factors associated with micro-ribonucleoproteins (microRNPs). We show that human microRNA miR369-3 directs association of these proteins with the AREs to activate translation. Furthermore, we document that two well-studied microRNAs-Let-7 and the synthetic microRNA miRcxcr4-likewise induce translation up-regulation of target mRNAs on cell cycle arrest, yet they repress translation in proliferating cells. Thus, activation is a common function of microRNPs on cell cycle arrest. We propose that translation regulation by microRNPs oscillates between repression and activation during the cell cycle.read more
Citations
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Journal ArticleDOI
Regulation of cytokines by small RNAs during skin inflammation
TL;DR: The nature of cytokines in psoriasis is reviewed with particular emphasis on regulation by mRNA destabilizing elements and the potential targeting of cytokine-encoding mRNAs by miRNAs, and the proposed linkage between mRNA decay mediated by AU-rich elements and miRNA association is described.
Journal ArticleDOI
MiR-92a promotes tumorigenesis of colorectal cancer, a transcriptomic and functional based study.
TL;DR: It is found miR-92a expression is associated with CRC pathological process and acts as an oncomir and directly targets Wnt/β-catenin, PTEN/Akt/FoxO, and BMP/Smads related genes, thus participates in CRC progression.
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Evidence for the complexity of microRNA-mediated regulation in ovarian cancer: a systems approach.
Shubin W. Shahab,Shubin W. Shahab,Lilya V. Matyunina,Lilya V. Matyunina,Roman Mezencev,Roman Mezencev,L. DeEtte Walker,L. DeEtte Walker,Nathan J. Bowen,Benedict B. Benigno,John F. McDonald,John F. McDonald +11 more
TL;DR: It is demonstrated that this low inverse correlation between changes in levels of miRNAs and their target mRNAs in vivo is not merely an artifact of inaccurate miRNA target predictions but the likely consequence of indirect cellular processes that modulate the regulatory effects of miRNA-mediated regulation in vivo.
Journal ArticleDOI
Identification of microRNA and bioinformatics target gene analysis in beef cattle intramuscular fat and subcutaneous fat.
TL;DR: This study provides the first evidence for better understanding the differential expression and mechanisms of miRNA in bovine fat deposition, and provides thinking to improve the quality of beef by reducing subcutaneous fat and increasing intramuscular fat of beef cattle.
Journal ArticleDOI
High throughput genome-wide survey of small RNAs from the parasitic protists Giardia intestinalis and Trichomonas vaginalis
TL;DR: The results indicate that miRNAs are likely to be general in ancestral eukaryotes and therefore arelikely to be a universal feature of eUKaryotes.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Control of translation and mRNA degradation by miRNAs and siRNAs
TL;DR: Possible mechanisms by which miRNAs control translation and mRNA degradation are discussed, an emerging theme is that mi RNAs, and siRNAs to some extent, target m RNAs to the general eukaryotic machinery for mRNA degradation and translation control.
Journal ArticleDOI
AU-rich elements: characterization and importance in mRNA degradation.
Chyi-Ying A. Chen,Ann-Bin Shyu +1 more
TL;DR: Observations suggest that Adenylate/uridylate-rich elements play a critical role in the regulation of gene expression during cell growth and differentiation and in the immune response.
Journal ArticleDOI
Specificity of microRNA target selection in translational repression
John G. Doench,Phillip A. Sharp +1 more
TL;DR: The ability of an miRNA to translationally repress a target mRNA is largely dictated by the free energy of binding of the first eight nucleotides in the 5' region of the miRNA, however, G:U wobble base-pairing in this region interferes with activity beyond that predicted on the basis of thermodynamic stability.
Journal ArticleDOI
From the U. S. A.
TL;DR: In this article, the existence of a monotone interpolating function with the same index set was shown to be necessary and sufficient for continuous interpolating functional if the index set I is finite, but not sufficient if I is infinite.
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