Journal ArticleDOI
Switching from repression to activation: microRNAs can up-regulate translation.
Reads0
Chats0
TLDR
It is proposed that translation regulation by microRNPs oscillates between repression and activation during the cell cycle, and two well-studied microRNAs—Let-7 and the synthetic microRNA miRcxcr4—likewise induce translation up-regulation of target mRNAs on cell cycle arrest.Abstract:
AU-rich elements (AREs) and microRNA target sites are conserved sequences in messenger RNA (mRNA) 3' untranslated regions (3'UTRs) that control gene expression posttranscriptionally. Upon cell cycle arrest, the ARE in tumor necrosis factor-alpha (TNFalpha) mRNA is transformed into a translation activation signal, recruiting Argonaute (AGO) and fragile X mental retardation-related protein 1 (FXR1), factors associated with micro-ribonucleoproteins (microRNPs). We show that human microRNA miR369-3 directs association of these proteins with the AREs to activate translation. Furthermore, we document that two well-studied microRNAs-Let-7 and the synthetic microRNA miRcxcr4-likewise induce translation up-regulation of target mRNAs on cell cycle arrest, yet they repress translation in proliferating cells. Thus, activation is a common function of microRNPs on cell cycle arrest. We propose that translation regulation by microRNPs oscillates between repression and activation during the cell cycle.read more
Citations
More filters
Journal ArticleDOI
Oncogenic microRNA-4534 regulates PTEN pathway in prostate cancer.
Hannah Nip,Altaf A. Dar,Sharanjot Saini,Melissa Colden,Shahryari Varahram,Harshika Chowdhary,Soichiro Yamamura,Yozo Mitsui,Yuichiro Tanaka,Taku Kato,Yutaka Hashimoto,Marisa Shiina,Priyanka Kulkarni,Pritha Dasgupta,Mitsuho Imai-Sumida,Z. Laura Tabatabai,Kirsten L. Greene,Guoren Deng,Rajvir Dahiya,Shahana Majid +19 more
TL;DR: Results show that depletion of miR-4534 in PCa induces a tumor suppressor phenotype partly through induction of PTEN, which has important implications for identifying and defining the role of new PTEN regulators such as microRNAs in prostate tumorigenesis.
Journal ArticleDOI
Classical and noncanonical functions of miRNAs in cancers
TL;DR: In this paper , the authors summarize key aspects of miRNA involvement in cancer, with a special focus on these lesser-studied mechanisms of action, including epigenetic mechanisms, transcriptional dysregulation, chemical modifications and editing, and alterations in miRNA biogenesis proteins.
Journal ArticleDOI
Transcriptional control of PAX4-regulated miR-144/451 modulates metastasis by suppressing ADAMs expression.
Jian Zhang,Xing Qin,Qiangling Sun,Hezhou Guo,Xiongyan Wu,F Xie,Qinyang Xu,Ming Yan,Jian Liu,Ze-Guang Han,Wantao Chen +10 more
TL;DR: This discovery suggests that a PAX4–miR-144/451–ADAMs axis regulates human epithelial cancer metastasis, thus opening up therapeutic possibilities and predicting prognosis for those cancer types.
Journal ArticleDOI
Data mining for miRNAs and their targets in the Triticeae.
TL;DR: MicroRNAs (miRNAs) and the mRNA targets of miRNAs were identified by sequence complementarity within a DNA sequence database for species of the Triticeae, providing further evidence of the existence of 26 miRNA families in the cereals.
Journal ArticleDOI
Tissular and soluble miRNAs for diagnostic and therapy improvement in digestive tract cancers.
TL;DR: Circulating miRNAs were demonstrated as valuable instruments in tumor diagnosis and the prognosis of digestive cancers, and are being investigated thoroughly in order to generate and validate less-invasive diagnostic tools with enhanced sensitivity.
References
More filters
疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Control of translation and mRNA degradation by miRNAs and siRNAs
TL;DR: Possible mechanisms by which miRNAs control translation and mRNA degradation are discussed, an emerging theme is that mi RNAs, and siRNAs to some extent, target m RNAs to the general eukaryotic machinery for mRNA degradation and translation control.
Journal ArticleDOI
AU-rich elements: characterization and importance in mRNA degradation.
Chyi-Ying A. Chen,Ann-Bin Shyu +1 more
TL;DR: Observations suggest that Adenylate/uridylate-rich elements play a critical role in the regulation of gene expression during cell growth and differentiation and in the immune response.
Journal ArticleDOI
Specificity of microRNA target selection in translational repression
John G. Doench,Phillip A. Sharp +1 more
TL;DR: The ability of an miRNA to translationally repress a target mRNA is largely dictated by the free energy of binding of the first eight nucleotides in the 5' region of the miRNA, however, G:U wobble base-pairing in this region interferes with activity beyond that predicted on the basis of thermodynamic stability.
Journal ArticleDOI
From the U. S. A.
TL;DR: In this article, the existence of a monotone interpolating function with the same index set was shown to be necessary and sufficient for continuous interpolating functional if the index set I is finite, but not sufficient if I is infinite.
Related Papers (5)
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets
MicroRNA expression profiles classify human cancers
Jun Lu,Gad Getz,Eric A. Miska,Eric A. Miska,Ezequiel Alvarez-Saavedra,Justin Lamb,David Peck,Alejandro Sweet-Cordero,Alejandro Sweet-Cordero,Benjamin L. Ebert,Benjamin L. Ebert,Raymond H. Mak,Raymond H. Mak,Adolfo A. Ferrando,James R. Downing,Tyler Jacks,H. Robert Horvitz,H. Robert Horvitz,Todd R. Golub,Todd R. Golub,Todd R. Golub +20 more