Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica.
TLDR
Synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast and human colon cancer cell lines.Abstract:
Using aqueous cyanobacterial extracts in the synthesis of silver nanoparticle is looked as green, ecofriendly, low priced biotechnology that gives advancement over both chemical and physical methods. In the current study, an aqueous extract of Oscillatoria limnetica fresh biomass was used for the green synthesis of Ag-NPs, since O. limnetica extract plays a dual part in both reducing and stabilizing Oscillatoria-silver nanoparticles (O-AgNPs). The UV-Visible absorption spectrum, Fourier transforms infrared (FT-IR), transmission electron microscopy (TEM) and scanning electron microscope (SEM) were achieved for confirming and characterizing the biosynthesized O-AgNPs. TEM images detected the quasi-spherical Ag-NPs shape with diverse size ranged within 3.30–17.97 nm. FT-IR analysis demonstrated the presence of free amino groups in addition to sulfur containing amino acid derivatives acting as stabilizing agents as well as the presence of either sulfur or phosphorus functional groups which possibly attaches silver. In this study, synthesized Ag-NPs exhibited strong antibacterial activity against multidrug-resistant bacteria (Escherichia coli and Bacillus cereus) as well as cytotoxic effects against both human breast (MCF-7) cell line giving IC50 (6.147 µg/ml) and human colon cancer (HCT-116) cell line giving IC50 (5.369 µg/ml). Hemolytic activity of Ag-NPs was investigated and confirmed as being non- toxic to human RBCs in low concentrations.read more
Citations
More filters
Journal ArticleDOI
Metal-based nanoparticles as antimicrobial agents: an overview.
Elena Sánchez-López,Elena Sánchez-López,Daniela Gomes,Gerard Esteruelas,Lorena Bonilla,Ana López-Machado,Ana López-Machado,Ruth Galindo,Amanda Cano,Amanda Cano,Marta Espina,Miren Ettcheto,Miren Ettcheto,Antoni Camins,Antoni Camins,Amélia M. Silva,Alessandra Durazzo,Antonello Santini,Maria Luisa García,Maria Luisa García,Eliana B. Souto,Eliana B. Souto +21 more
TL;DR: Their added-value in the development of alternative, more effective antibiotics against multi-resistant Gram-negative bacteria has been highlighted and their production methods, physicochemical characterization, and pharmacokinetics are reviewed.
Journal ArticleDOI
Silver nanoparticles: Synthesis, medical applications and biosafety
TL;DR: The state-of-the-art advances of AgNPs in the synthesis methods, medical applications and biosafety, and a new type of Ag particles, silver Ångstrom (Å, 1 Å = 0.1 nm) particles (AgÅPs), which exhibit better biological activity and lower toxicity compared with AgNps are reviewed.
Journal ArticleDOI
Green Synthesis of Metallic Nanoparticles and Their Potential Applications to Treat Cancer.
TL;DR: This review covers the green synthesis of nanoparticles by using various biological systems as well as their applications, which have a range of unlimited pharmaceutical applications including delivery of drugs or genes, detection of pathogens or proteins, and tissue engineering.
Journal ArticleDOI
Silver Nanoparticles: Mechanism of Action and Probable Bio-Application
TL;DR: This review is devoted to the medical application of silver nanoparticles produced as a result of “green” synthesis using various living organisms (bacteria, fungi, plants).
Journal ArticleDOI
Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics
Salem S. Salem,Ehab F. El-Belely,Gniewko Niedbała,Maryam M. Alnoman,Saad El-Din Hassan,Ahmed M. Eid,Tharwat I. Shaheen,Amr Elkelish,Amr Fouda +8 more
TL;DR: The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at different concentrations against the pathogens Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhimurium and exhibited more activity against pathogenic Gram-positive and Gram-negative bacteria, indicating the stability of the treated fabrics.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal ArticleDOI
Systematic Review: Process of Forming Academic Service Partnerships to Reform Clinical Education
TL;DR: This study’s findings can provide practical guidelines to steer partnership programs within the academic and clinical bodies, with the aim of providing a collaborative partnership approach to clinical education.
Journal ArticleDOI
Antibiotic susceptibility testing by a standardized single disk method.
TL;DR: Recommendations of the National Committee for Clinical Laboratory Standards continue to be based on this publication; the “Kirby-Bauer” method is, among the many disk methods used in other countries, still the one that has been researched most thoroughly and updated continuously.
Journal ArticleDOI
Generic assignments, strain histories, and properties of pure cultures of cyanobacteria
TL;DR: Revisions are designed to permit the generic identification of cultures, often difficult through use of the field-based system of phycological classification, and are both constant and readily determinable in cultured material.