The Expression and Nuclear Deposition of Histone H3.1 in Murine Oocytes and Preimplantation Embryos
TLDR
Results suggest that H3.1 is not incorporated into chromatin due to the inactivity of the histone chaperone and low mRNA expression level, which suggests that chromatin remodeling that takes place during development is not included.Abstract:
Differentiated oocytes acquire totipotency through fertilization. During this transition, genome-wide chromatin remodeling occurs, which leads to change in gene expression. However, the mechanism that underlies this global change in chromatin structure has not been fully elucidated. Histone variants play a key role in defining chromatin structure and are implicated in inheritance of epigenetic information. In this study, we analyzed the nuclear localization and expression of H3.1 to elucidate the role of this histone variant in chromatin remodeling during oogenesis and preimplantation development. Analysis using Flag-tagged H3.1 transgenic mice revealed that Flag-H3.1 was not present in differentiated oocytes or early preimplantation embryos before the morula stage, although Flag-H3.1 mRNA was expressed at all stages examined. In addition, the expression levels of endogenous H3.1 genes were low at the stages where H3.1 was not present in chromatin. These results suggest that H3.1 is not incorporated into chromatin due to the inactivity of the histone chaperone and low mRNA expression level. The significance of the dynamics of H3.1 is evaluated in terms of chromatin remodeling that takes place during development.read more
Citations
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Developmental roles of histone H3 variants and their chaperones.
Dan Filipescu,Dan Filipescu,Emmanuelle Szenker,Emmanuelle Szenker,Geneviève Almouzni,Geneviève Almouzni +5 more
TL;DR: The importance of the H3.3 replacement variant for the nuclear reprogramming that occurs during gametogenesis, fertilization, and germline establishment is highlighted and the challenges of maintaining centromeric identity through propagation of the Centromeric CenH3 variant in different cell types are discussed.
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Histone H3 Variants and Their Chaperones During Development and Disease: Contributing to Epigenetic Control
TL;DR: The importance of H3 variants in the context of nuclear reprogramming and cell differentiation is integrated, and a case in which the identity of a given genomic locus is propagated across different cell types is described.
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Expression and functionality of histone H2A variants in cancer
Fátima Liliana Monteiro,Tiago Baptista,Francisco Amado,Rui Vitorino,Carmen Jerónimo,Luisa A. Helguero +5 more
TL;DR: Investigation of gene expression includes the replacement of canonical histones for non-allelic histone variants, as well as their multiple targeting by postranslational modifications, to understand histone H2A variants, their contribution to normal cellular function and to cancer development and progression.
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Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons
Yuki Hatanaka,Kimiko Inoue,Mami Oikawa,Satoshi Kamimura,Narumi Ogonuki,Eiichi Kodama,Yasuyuki Ohkawa,Yu Ichi Tsukada,Atsuo Ogura,Atsuo Ogura +9 more
TL;DR: Hypomethylated preimplantation mouse embryos are protected from retrotransposons by repressive histone modifications mediated by the histone chaperone chromatin assembly factor 1 (CAF-1), indicating that CAF- 1 is an essential guardian of the genome in preim implantation mice embryos by deposition of repressivehistone modifications via histone variant replacement.
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