The Role of Vimentin Intermediate Filaments in Cortical and Cytoplasmic Mechanics
Ming Guo,Allen J. Ehrlicher,Allen J. Ehrlicher,Saleemulla Mahammad,Hilary Fabich,Mikkel H. Jensen,Mikkel H. Jensen,Jeffrey R. Moore,Jeffrey J. Fredberg,Robert D. Goldman,David A. Weitz +10 more
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TLDR
Studying mouse embryonic fibroblasts derived from wild-type or vimentin(-/-) mice shows that VIFs both increase the mechanical integrity of cells and localize intracellular components.About:
This article is published in Biophysical Journal.The article was published on 2013-10-01 and is currently open access. It has received 230 citations till now. The article focuses on the topics: Cytoskeleton & Intermediate filament.read more
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Probing the Stochastic, Motor-Driven Properties of the Cytoplasm Using Force Spectrum Microscopy
Ming Guo,Allen J. Ehrlicher,Allen J. Ehrlicher,Mikkel H. Jensen,Mikkel H. Jensen,Malte Renz,Jeffrey R. Moore,Robert D. Goldman,Jennifer Lippincott-Schwartz,Fred C. MacKintosh,David A. Weitz +10 more
TL;DR: This work introduces force-spectrum-microscopy (FSM) to directly quantify random forces within the cytoplasm of cells and thereby probe stochastic motor activity, and shows that force fluctuations substantially enhance intracellular movement of small and large components.
Journal ArticleDOI
Impact of the physical microenvironment on tumor progression and metastasis.
TL;DR: A new appreciation for the role of mechanics in cancer is emerging because in vitro systems are becoming increasingly sophisticated in differentiating between distinct physical cues-ECM pore size, fiber alignment, and molecular composition-and elucidating the different roles these properties play in driving tumor progression and metastasis.
Journal ArticleDOI
Cell volume change through water efflux impacts cell stiffness and stem cell fate.
Ming Guo,Adrian F. Pegoraro,Angelo S. Mao,Enhua H. Zhou,Praveen R. Arany,Yu Long Han,Yu Long Han,Dylan T. Burnette,Mikkel H. Jensen,Mikkel H. Jensen,Karen E. Kasza,Karen E. Kasza,Jeffrey R. Moore,Fred C. MacKintosh,Fred C. MacKintosh,Jeffrey J. Fredberg,David J. Mooney,Jennifer Lippincott-Schwartz,David A. Weitz +18 more
TL;DR: A robust and unified relationship between cell stiffness and cell volume is identified and it is found that changes in cell volume, and hence stiffness, alter stem-cell differentiation, regardless of the method by which these are induced.
Journal ArticleDOI
The molecular architecture of lamins in somatic cells
Yagmur Turgay,Matthias Eibauer,Anne E. Goldman,Takeshi Shimi,Maayan Khayat,Kfir Ben-Harush,Anna Dubrovsky-Gaupp,K. Tanuj Sapra,Robert D. Goldman,Ohad Medalia,Ohad Medalia +10 more
TL;DR: In this article, a detailed view of the organization of the lamin meshwork within the lamina was obtained using cryo-electron tomography, which showed that A-and B-type lamins assemble into tetrameric filaments of 3.5 nm thickness.
Journal ArticleDOI
The soft mechanical signature of glial scars in the central nervous system.
Emad Moeendarbary,Emad Moeendarbary,Emad Moeendarbary,Isabell P. Weber,Graham K. Sheridan,Graham K. Sheridan,David E. Koser,Sara Soleman,Barbara Haenzi,Elizabeth J. Bradbury,James W. Fawcett,Kristian Franze +11 more
TL;DR: In this paper, the authors characterized spatiotemporal changes of the elastic stiffness of the injured rat neocortex and spinal cord at 1.5 and three weeks post-injury using atomic force microscopy.
References
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Vimentin induces changes in cell shape, motility, and adhesion during the epithelial to mesenchymal transition
TL;DR: Vimentin is used widely as a marker of the epithelial to mesenchymal transitions that take place during embryogenesis and metastasis, yet the functional implications of the expression of this type III intermediate filament (IF) protein are poorly understood.
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Mechanobiology and diseases of mechanotransduction
TL;DR: The key roles that physical forces, extracellular matrix and cell structure play in the control of normal development, as well as in the maintenance of tissue form and function are reviewed.
Journal ArticleDOI
Viscoelastic properties of vimentin compared with other filamentous biopolymer networks.
TL;DR: Parallel measurements of the viscoelasticity of tubulin, actin, and vimentin polymers are described and suggest possible specialized roles for the different classes of filaments in vivo.
Journal ArticleDOI
Microrheology of Human Lung Epithelial Cells Measured by Atomic Force Microscopy
Jordi Alcaraz,Lara Buscemi,Mireia Grabulosa,Xavier Trepat,Ben Fabry,Ramon Farré,Daniel Navajas +6 more
TL;DR: This complex dynamics is consistent with the rheology of soft glassy materials close to a glass transition, thereby suggesting that structural disorder and metastability may be fundamental features of cell architecture.
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Intermediate filaments: from cell architecture to nanomechanics
TL;DR: The analysis of these various diseases suggests that IFs also have an important role in cell-type-specific physiological functions, and the nanomechanical properties of cell- type-specific IFs are central to the pathogenesis of diseases as diverse as muscular dystrophy and premature ageing.