The Role of Vimentin Intermediate Filaments in Cortical and Cytoplasmic Mechanics
Ming Guo,Allen J. Ehrlicher,Allen J. Ehrlicher,Saleemulla Mahammad,Hilary Fabich,Mikkel H. Jensen,Mikkel H. Jensen,Jeffrey R. Moore,Jeffrey J. Fredberg,Robert D. Goldman,David A. Weitz +10 more
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TLDR
Studying mouse embryonic fibroblasts derived from wild-type or vimentin(-/-) mice shows that VIFs both increase the mechanical integrity of cells and localize intracellular components.About:
This article is published in Biophysical Journal.The article was published on 2013-10-01 and is currently open access. It has received 230 citations till now. The article focuses on the topics: Cytoskeleton & Intermediate filament.read more
Citations
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Advances in the microrheology of complex fluids.
TL;DR: The requirements for a simple modern particle tracking microrheology experiment are introduced, the error analysis methods associated with it and the mathematical techniques required to calculate the linear viscoelasticity are discussed.
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Vimentin Enhances Cell Elastic Behavior and Protects against Compressive Stress
TL;DR: The results show that the Young's moduli of normal and vim(-/-)mEFs are substrate stiffness dependent even when the spread area is similar, and that vimentin protects against compressive stress and preserves mechanical integrity by enhancing cell elastic behavior.
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Cytoskeletal dynamics: a view from the membrane.
TL;DR: These studies highlight the membrane’s central role in coordinating these cytoskeletal systems to carry out essential processes, such as endocytosis, spindle positioning, and cellular compartmentalization.
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Cell stiffness determined by atomic force microscopy and its correlation with cell motility
TL;DR: This review provides an overview of the current understanding of the correlation between AFM-determined cell stiffness and motility, the determinants of this detecting method, as well as clues to optimize detecting parameters.
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Vimentin protects cells against nuclear rupture and DNA damage during migration
Alison E. Patteson,Alison E. Patteson,Amir Vahabikashi,Katarzyna Pogoda,Katarzyna Pogoda,Stephen A. Adam,Koushik Mandal,Mark Kittisopikul,Suganya Sivagurunathan,Anne E. Goldman,Robert D. Goldman,Paul A. Janmey +11 more
TL;DR: It is demonstrated that vimentin intermediate filaments in mesenchymal cells form a cage-like filamentous network around the nucleus that protects the cell against extreme nucleus deformations, rupture, and DNA damage during migration in constricted 3D spaces.
References
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Cell mechanics and the cytoskeleton
TL;DR: An important insight emerging from this work is that long-lived cytoskeletal structures may act as epigenetic determinants of cell shape, function and fate.
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Mechanotransduction at a distance: mechanically coupling the extracellular matrix with the nucleus
TL;DR: The molecular mechanisms by which forces might act at a distance to induce mechanochemical conversion in the nucleus and alter gene activities are explored.
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Scaling the microrheology of living cells.
Ben Fabry,Geoffrey N. Maksym,James P. Butler,Michael Glogauer,Daniel Navajas,Jeffrey J. Fredberg +5 more
TL;DR: A scaling law is reported that governs both the elastic and frictional properties of a wide variety of living cell types, over a wide range of time scales and under a variety of biological interventions, and implies that cytoskeletal proteins may regulate cell mechanical properties mainly by modulating the effective noise temperature of the matrix.
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Nonequilibrium mechanics of active cytoskeletal networks.
Daisuke Mizuno,Catherine Tardin,Christoph F. Schmidt,Christoph F. Schmidt,Fred C. MacKintosh +4 more
TL;DR: A quantitative theoretical model is presented connecting the large-scale properties of this active gel to molecular force generation and qualitatively changing the viscoelastic response of the network in an adenosine triphosphate–dependent manner.