The VELVET A orthologue VEL1 of Trichoderma reesei regulates fungal development and is essential for cellulase gene expression.
Razieh Karimi Aghcheh,Zoltán Németh,Lea Atanasova,Erzsébet Fekete,Melinda Paholcsek,Erzsébet Sándor,Benigno Aquino,Irina S. Druzhinina,Levente Karaffa,Christian P. Kubicek +9 more
TLDR
Deletion of vel1 completely impaired the expression of cellulases, xylanases and the cellulase regulator XYR1 on lactose as a cellulase inducing carbon source, but also in resting mycelia with sophorose as inducer.Abstract:
Trichoderma reesei is the industrial producer of cellulases and hemicellulases for biorefinery processes. Their expression is obligatorily dependent on the function of the protein methyltransferase LAE1. The Aspergillus nidulans orthologue of LAE1 - LaeA - is part of the VELVET protein complex consisting of LaeA, VeA and VelB that regulates secondary metabolism and sexual as well as asexual reproduction. Here we have therefore investigated the function of VEL1, the T. reesei orthologue of A. nidulans VeA. Deletion of the T. reesei vel1 locus causes a complete and light-independent loss of conidiation, and impairs formation of perithecia. Deletion of vel1 also alters hyphal morphology towards hyperbranching and formation of thicker filaments, and with consequently reduced growth rates. Growth on lactose as a sole carbon source, however, is even more strongly reduced and growth on cellulose as a sole carbon source eliminated. Consistent with these findings, deletion of vel1 completely impaired the expression of cellulases, xylanases and the cellulase regulator XYR1 on lactose as a cellulase inducing carbon source, but also in resting mycelia with sophorose as inducer. Our data show that in T. reesei VEL1 controls sexual and asexual development, and this effect is independent of light. VEL1 is also essential for cellulase gene expression, which is consistent with the assumption that their regulation by LAE1 occurs by the VELVET complex.read more
Citations
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VelB/VeA/LaeA Complex CoordinatesLight Signal with Fungal Developmentand Secondary Metabolism
Özgür Bayram,Sven Krappmann,Min Ni,Jin Woo Bok,Kerstin Helmstaedt,Oliver Valerius,Susanna A. Braus-Stromeyer,Nak-Jung Kwon,Nancy P. Keller,Jae-Hyuk Yu,Gerhard H. Braus +10 more
TL;DR: This work has identified the heterotrimeric velvet complex VelB/VeA/LaeA connecting light-responding developmental regulation and control of secondary metabolism in Aspergillus nidulans, which inhibits sexual reproduction as well as secondary metabolism.
Journal ArticleDOI
Cellulases and beyond: the first 70 years of the enzyme producer Trichoderma reesei.
TL;DR: This review summarizes important milestones of the development of T. reesei as the leading production host for biorefinery enzymes, and discusses emerging trends in strain engineering of the filamentous ascomycete TrichodermaReesei.
Journal ArticleDOI
A critical review of clay-based composites with enhanced adsorption performance for metal and organic pollutants.
TL;DR: The comparison of clay composites used for the removal of organic and inorganic contaminants provides valuable insight into real wastewater treatment and knowledge gaps, uncertainties, and future challenges involved in the fabrication and regeneration of modified clay Composites are identified.
Journal ArticleDOI
Regulators of plant biomass degradation in ascomycetous fungi.
Tiziano Benocci,Maria Victoria Aguilar-Pontes,Miaomiao Zhou,Bernhard Seiboth,Ronald P. de Vries +4 more
TL;DR: This review will summarize the current knowledge on regulation of plant biomass utilization in fungi and compare the differences between fungal species, focusing in particular on the presence or absence of the regulators involved in this process.
Journal ArticleDOI
Genetic engineering of Trichoderma reesei cellulases and their production
TL;DR: The structure and mechanism of the enzymes involved, the regulation of their expression and the pathways of their formation and secretion have been investigated in T. reesei in considerable details and several of the findings have been used to improve the formation of the T.Reesei cellulases and their properties.
References
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