Titanium dioxide nanoparticles exacerbate DSS-induced colitis: role of the NLRP3 inflammasome
Pedro A. Ruiz,Belén Morón,Helen M. Becker,Silvia Lang,Kirstin Atrott,Marianne R. Spalinger,Michael Scharl,Kacper A. Wojtal,Anne Fischbeck-Terhalle,Isabelle Frey-Wagner,Martin Hausmann,Thomas Kraemer,Gerhard Rogler +12 more
Reads0
Chats0
TLDR
Findings indicate that individuals with a defective intestinal barrier function and pre-existing inflammatory condition, such as IBD, might be negatively impacted by the use of TiO2 nanoparticles.Abstract:
Objective Western lifestyle and diet are major environmental factors playing a role in the development of IBD. Titanium dioxide (TiO 2 ) nanoparticles are widely used as food additives or in pharmaceutical formulations and are consumed by millions of people on a daily basis. We investigated the effects of TiO 2 in the development of colitis and the role of the nucleotide-binding oligomerisation domain receptor, pyrin domain containing (NLRP)3 inflammasome. Design Wild-type and NLRP3-deficient mice with dextran sodium sulfate-induced colitis were orally administered with TiO 2 nanoparticles. The proinflammatory effects of TiO 2 particles in cultured human intestinal epithelial cells (IECs) and macrophages were also studied, as well as the ability of TiO 2 crystals to traverse IEC monolayers and accumulate in the blood of patients with IBD using inductively coupled plasma mass spectrometry. Results Oral administration of TiO 2 nanoparticles worsened acute colitis through a mechanism involving the NLRP3 inflammasome. Importantly, crystals were found to accumulate in spleen of TiO 2 -administered mice. In vitro, TiO 2 particles were taken up by IECs and macrophages and triggered NLRP3-ASC-caspase-1 assembly, caspase-1 cleavage and the release of NLRP3-associated interleukin (IL)-1β and IL-18. TiO 2 also induced reactive oxygen species generation and increased epithelial permeability in IEC monolayers. Increased levels of titanium were found in blood of patients with UC having active disease. Conclusion These findings indicate that individuals with a defective intestinal barrier function and pre-existing inflammatory condition, such as IBD, might be negatively impacted by the use of TiO 2 nanoparticles.read more
Citations
More filters
Journal ArticleDOI
Pharmacological Basis for Use of a Novel Compound in Hyperuricemia: Anti-Hyperuricemic and Anti-Inflammatory Effects
Lei Zhao,Yihang Li,Dahong Yao,Ran Sun,Liu Shifang,Xi Chen,Cong-Cong Lin,Jian Huang,Jin-Hui Wang,Guang Li +9 more
TL;DR: In this article, the effects of the novel compound on lowering the serum uric acid level and alleviating renal inflammation induced by high urological acid in hyperuricemic mice were investigated.
Journal ArticleDOI
Combined effect of titanium dioxide nanoparticles and glucose on the blood glucose homeostasis in young rats after oral administration.
TL;DR: It is found that subchronic co-exposure of TiO2 NPs and glucose caused slight imbalance of blood glucose homeostasis in vivo, which may be due to the effects of excess glucose and the interactions between TiO1 Nps and glucose.
Journal ArticleDOI
Changes of physico-chemical properties of nano-biomaterials by digestion fluids affect the physiological properties of epithelial intestinal cells and barrier models
Giulia Antonello,Arianna Marucco,Elena Gazzano,Panagiotis Kainourgios,Costanza Ravagli,Ana González-Paredes,Simone Sprio,Esperanza Padín-González,Mahmoud Soliman,David Béal,Francesco Barbero,P Gasco,Giovanni Baldi,Marie Carrière,Marco P. Monopoli,Costas A. Charitidis,Enrico Bergamaschi,Ivana Fenoglio,Chiara Riganti +18 more
TL;DR: In this paper , nano-biomaterials (NBMs) were evaluated on primary (HCoEpiC) and immortalised (Caco-2, HCT116) epithelial intestinal cells and on an intestinal barrier model.
Journal ArticleDOI
The consequences of particle uptake on immune cells.
TL;DR: In this article , the authors review the recent literature that describes the changes induced in immune cells following internalization of cargo-free particles and discuss how the inherent immunomodulatory properties of particles may be leveraged for therapeutic applications.
Journal ArticleDOI
Chronic exposure to synthetic food colorant Allura Red AC promotes susceptibility to experimental colitis via intestinal serotonin in mice
Yun Han Kwon,Suhrid Banskota,Huaqing Wang,Laura Rossi,Jensine Grondin,Saad A. Syed,Y. Yousefi,Jonathan D. Schertzer,Katherine M. Morrison,Michael G. Wade,Alison C. Holloway,Michael G. Surette,Gregory R. Steinberg,Waliul I. Khan +13 more
TL;DR: The authors showed that chronic long-term exposure to a common synthetic colorant promotes experimental colitis via colonic 5-HT in a gut microbiota-dependent and independent pathway in mice.
References
More filters
Journal ArticleDOI
Gout-associated uric acid crystals activate the NALP3 inflammasome
TL;DR: It is shown that MSU and CPPD engage the caspase-1-activating NALP3 (also called cryopyrin) inflammasome, resulting in the production of active interleukin (IL)-1β and IL-18 in mice deficient in the IL-1β receptor.
Journal ArticleDOI
Clinical epidemiology of inflammatory bowel disease: incidence, prevalence, and environmental influences
TL;DR: Differences in incidence across age, time, and geographic region suggest that environmental factors significantly modify the expression of Crohn's disease and ulcerative colitis.
Journal ArticleDOI
Cryopyrin activates the inflammasome in response to toxins and ATP
Sanjeev Mariathasan,David S. Weiss,Kim Newton,Jacqueline McBride,Karen O'Rourke,Meron Roose-Girma,Wyne P. Lee,Yvette Weinrauch,Denise M. Monack,Vishva M. Dixit +9 more
TL;DR: It is shown that cryopyrin-deficient macrophages cannot activate caspase-1 in response to Toll-like receptor agonists plus ATP, the latter activating the P2X7 receptor to decrease intracellular K+ levels.
Journal ArticleDOI
Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization
Veit Hornung,Franz Bauernfeind,Annett Halle,Eivind O. Samstad,Eivind O. Samstad,Hajime Kono,Kenneth L. Rock,Katherine A. Fitzgerald,Eicke Latz,Eicke Latz +9 more
TL;DR: It is demonstrated that silica and aluminum salt crystals activated inflammasomes formed by the cytoplasmic receptor NALP3, which senses lysosomal damage as an endogenous 'danger' signal.
Related Papers (5)
Food-grade TiO 2 impairs intestinal and systemic immune homeostasis, initiates preneoplastic lesions and promotes aberrant crypt development in the rat colon.
Sarah Bettini,Elisa Boutet-Robinet,Christel Cartier,Christine Coméra,Eric Gaultier,J. Dupuy,Nathalie Naud,Sylviane Taché,Patrick Grysan,Solenn Reguer,Nathalie Thieriet,Matthieu Réfrégiers,Dominique Thiaudière,Jean Pierre Cravedi,Marie Carrière,Jean-Nicolas Audinot,Fabrice Pierre,Laurence Guzylack-Piriou,Eric Houdeau +18 more