Transcriptional profiling of Mycoplasma hyopneumoniae during heat shock using microarrays
TLDR
91 genes that had significant transcriptional differences in response to heat shock conditions were identified and many of the heat shock proteins previously characterized in other bacteria were identified as significant in this study as well.Abstract:
Bacterial pathogens undergo stress during host colonization and disease processes. These stresses result in changes in gene expression to compensate for potentially lethal environments developed in the host during disease. Mycoplasma hyopneumoniae colonizes the swine epithelium and causes a pneumonia that predisposes the host to enhanced disease from other pathogens. How M. hyopneumoniae responds to changing environments in the respiratory tract during disease progression is not known. In fact, little is known concerning the capabilities of mycoplasmas to respond to changing growth environments. With limited genes, mycoplasmas are thought to possess only a few mechanisms for gene regulation. A microarray consisting of 632 of the 698 open reading frames of M. hyopneumoniae was constructed and used to study gene expression differences during a temperature shift from 37°C to 42°C, a temperature swing that might be encountered during disease. To enhance sensitivity, a unique hexamer primer set was employed for generating cDNA from only mRNA species. Our analysis identified 91 genes that had significant transcriptional differences in response to heat shock conditions (P < 0.01) with an estimated false-discovery rate of 4 percent. Thirty-three genes had a change threshold of 1.5-fold or greater. Many of the heat shock proteins previously characterized in other bacteria were identified as significant in this study as well. A proportion of the identified genes (54 of 91) currently have no assigned function.read more
Citations
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Journal ArticleDOI
Effects of temperature on gene expression patterns in Leptospira interrogans serovar lai as assessed by whole-genome microarrays
Miranda Lo,Dieter M. Bulach,David R. Powell,David A. Haake,David A. Haake,James Matsunaga,James Matsunaga,Michael L. Paustian,Richard L. Zuerner,Ben Adler,Ben Adler +10 more
TL;DR: Temperature is an important factor regulating expression of proteins that facilitate invasion and establishment of disease in Leptospira spp.
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Repeat regions R1 and R2 in the P97 paralogue Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin and porcine cilia
Ania T. Deutscher,Cheryl Jenkins,F. Chris Minion,Lisa M. Seymour,Matthew P. Padula,Nicholas E. Dixon,Mark J. Walker,Steven P. Djordjevic +7 more
TL;DR: Rec recombinant proteins containing R1A271 and R2271‐R1B271 were constructed and used in in vitro binding assays to suggest that both R1 and R2 in Mhp271 are involved in binding to host molecules.
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Tools for the genetic analysis of Mycoplasma.
Sven Halbedel,Jörg Stülke +1 more
TL;DR: An overview on recent developments in Mycoplasma genetics that facilitate the genetic manipulation of these interesting organisms is given.
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Proteomic survey of the pathogenic Mycoplasma hyopneumoniae strain 7448 and identification of novel post-translationally modified and antigenic proteins.
Paulo Marcos Pinto,Gustavo Chemale,Luiza Amaral de Castro,Ana Paula Metz Costa,Jalusa Deon Kich,Marilene Henning Vainstein,Arnaldo Zaha,Henrique Bunselmeyer Ferreira +7 more
TL;DR: A proteomic analysis, based on two-dimensional gel electrophoresis of soluble protein extracts, immunoblot and mass spectrometry, which was carried out aiming the identification of gene products and antigenic proteins from the M. hyopneumoniae pathogenic strain 7448 produced a proteome map that is expected to serve as a reference for comparative analyses for methabolic studies based on cells cultured under modified conditions.
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Global transcriptional analysis of Mycoplasma hyopneumoniae following exposure to norepinephrine.
TL;DR: In response to norepinephrine, M. hyopneumoniae appears to upregulate protein expression while downregulating general metabolism, which correlated with the reduction in growth of the mycoplasma.
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