Ultrastructural view of astrocyte arborization, astrocyte-astrocyte and astrocyte-synapse contacts, intracellular vesicle-like structures, and mitochondrial network
Sydney Aten,Conrad M. Kiyoshi,Emily P. Arzola,Jeremy A. Patterson,Anne T. Taylor,Yixing Du,Ally M. Guiher,Merna Philip,Elizabeth Gerviacio Camacho,Devin Mediratta,Kelsey A Collins,K. De Boni,Silvana A. Garcia,Rajesh Kumar,Aiden N. Drake,Ahlam Hegazi,Lindsey Trank,Emily Benson,Grahame J. Kidd,David Terman,Ming Zhou +20 more
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TLDR
In this paper , the authors trace and 3D reconstruct three neighboring astrocytes using serial block-face scanning electron microscopy and reveal a spongiform morphology resulting from the abundance of reflexive and leaflet processes.About:
This article is published in Progress in Neurobiology.The article was published on 2022-03-01 and is currently open access. It has received 35 citations till now. The article focuses on the topics: Medicine & Astrocyte.read more
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A closer look at astrocyte morphology: Development, heterogeneity, and plasticity at astrocyte leaflets
TL;DR: Astrocytes represent an abundant type of glial cells involved in nearly every aspect of central nervous system (CNS) function, including synapse formation and maturation, ion and neurotransmitter homeostasis, blood-brain barrier maintenance, as well as neuronal metabolic support as discussed by the authors .
Posted ContentDOI
Organizing Principles of Astrocytic Nanoarchitecture in the Mouse Cerebral Cortex
Christopher K. Salmon,Tabish A. Syed,J. Benjamin Kacerovsky,Nensi Alivodej,Alexandra L. Schober,Michael T. Pratte,Michael P. Rosen,Miranda Green,Adario DasGupta,Hojatollah Vali,Craig A. Mandato,Kaleem Siddiqi,Keith K. Murai +12 more
TL;DR: In this article, high-resolution datasets of layer 2/3 mouse somatosensory cortex using focused ion beam scanning electron microscopy (8nm intervals) and computer vision approaches were used to provide a principled, quantitative analysis of astrocytic nanoarchitecture.
Journal ArticleDOI
Chronic Stress Impairs the Structure and Function of Astrocyte Networks in an Animal Model of Depression
Sydney Aten,Yixing Du,Olivia Taylor,Courtney N. Dye,Kelsey A Collins,Matthew Thomas,Conrad M. Kiyoshi,Ming Zhou +7 more
TL;DR: Findings reveal that chronic stress leads toAstrocyte atrophy and impaired gap junction coupling, raising the prospect that both individual and network-level astrocytes functionality are important in the etiology of major depression and other neuropsychiatric disorders.
Journal ArticleDOI
Ketogenic diet uncovers differential metabolic plasticity of brain cells
Tim Düking,Lena Spieth,Stefan A. Berghoff,Lars Piepkorn,Annika M Schmidke,Miso Mitkovski,Nirmal Kannaiyan,Leon Hosang,Patricia Scholz,Ali H Shaib,Lennart V. Schneider,Dörte Hesse,Torben Ruhwedel,Ting Sun,Lisa Linhoff,Andrea Trevisiol,Susanne Köhler,Adrian Marti Pastor,Thomas Misgeld,Michael W. Sereda,Imam Hassouna,Moritz J. Rossner,Francesca Odoardi,Till Ischebeck,Livia de Hoz,Johannes Hirrlinger,Olaf Jahn,Gesine Saher +27 more
TL;DR: A proteome atlas of the major central nervous system cell types from young and adult mice is generated, after feeding the therapeutically relevant low-carbohydrate, high-fat ketogenic diet and during neuroinflammation to highlight the importance of metabolic cross-talk between CNS cells and between the periphery and the brain to manage altered nutrition and neurological disease.
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Structural Heterogeneity of the GABAergic Tripartite Synapse
TL;DR: Super-resolution expansion microscopy is established of GABAergic synapses and nearby astrocytic processes and their structural arrangements in the stratum radiatum of the mouse hippocampal CA1 region to speculate that this specific arrangement enables more efficient re-uptake of GABA into presynaptic terminals.
References
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Fiji: an open-source platform for biological-image analysis
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Tripartite synapses : Glia, the unacknowledged partner
TL;DR: It is suggested that perisynaptic Schwann cells and synaptically associated astrocytes should be viewed as integral modulatory elements of tripartite synapses.
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Brain energy metabolism: focus on astrocyte-neuron metabolic cooperation
TL;DR: This review focuses on the cellular aspects of brain energy metabolism, with a particular emphasis on the metabolic interactions between neurons and astrocytes.
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Serial block−face scanning electron microscopy to reconstruct three−dimensional tissue nanostructure
Winfried Denk,Heinz Horstmann +1 more
TL;DR: It is demonstrated that datasets meeting these requirements can be obtained by automated block-face imaging combined with serial sectioning inside the chamber of a scanning electron microscope, opening the possibility of automatically obtaining the electron-microscope-level 3D datasets needed to completely reconstruct the connectivity of neuronal circuits.