Journal ArticleDOI
Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
Shane T. Kenny,Jasmina Nikodinovic Runic,Walter Kaminsky,Trevor Woods,Ramesh Babu,Chris M. Keely,Werner J. Blau,Kevin E. O’Connor +7 more
TLDR
The conversion of the petrochemical polymer polyethylene terephthalate to a biodegradable plastic polyhydroxyal-kanoate (PHA) is described here and PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium.Abstract:
The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 °C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. freder...read more
Citations
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Journal ArticleDOI
Recycling of bioplastic waste: A review
Giulia Fredi,Andrea Dorigato +1 more
TL;DR: In this article, an end-of-life strategy for biobased non-biodegradable bioplastics such as bio-polyethylene terephthalate (bioPET), bio-Polyethylene (biioPE), and biopolypropylene (bIOPP) is discussed.
Journal ArticleDOI
Transdisciplinary Research in Sustainable Operations – An Application to Closed-Loop Supply Chains
TL;DR: In this paper, the authors identify the need for transdisciplinary collaboration within two steps: first, a literature review points out that various research disciplines as well as practice mostly operate in isolation, and then they develop a framework that highlights the benefits of collaboration between these research areas.
Journal ArticleDOI
Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology.
TL;DR: An overview of recent progress in studies on biobased polyesters, especially those made from short building blocks, synthesized through step-growth polymerization, and some important technical aspects of their syntheses through biotic or abiotic pathways are detailed.
Journal ArticleDOI
The metabolic potential of plastics as biotechnological carbon sources – Review and targets for the future
TL;DR: In this article , the authors summarized well-known monomer degradation pathways and computed their theoretical yields for industrially interesting products, and calculated replacement scenarios of existing fossil-based synthesis routes for the same products.
Journal ArticleDOI
Ketone Body 3-Hydroxybutyrate Ameliorates Atherosclerosis via Receptor Gpr109a-Mediated Calcium Influx
Zhang Shujie,Li Zihua,Zhang Yudian,Jin Chen,Yuan Li,Fuqing Wu,Wei Wang,Zong Jie Cui,Guo-Qiang Chen +8 more
TL;DR: Examination of the impact of an endogenous metabolite, namely ketone body 3‐hydroxybutyrate (3‐HB), on a mouse model of Atherosclerosis finds that daily oral administration of 3‐HB can significantly ameliorate atherosclerosis.
References
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Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
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Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.
A J Anderson,E A Dawes +1 more
TL;DR: The physiological functions of PHB as a reserve material and in symbiotic nitrogen fixation and its presence in bacterial plasma membranes and putative role in transformability and calcium signaling are also considered.
Journal ArticleDOI
A rapid gas chromatographic method for the determination of poly- β -hydroxybutyric acid in microbial biomass
TL;DR: The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l.
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