Journal ArticleDOI
Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
Shane T. Kenny,Jasmina Nikodinovic Runic,Walter Kaminsky,Trevor Woods,Ramesh Babu,Chris M. Keely,Werner J. Blau,Kevin E. O’Connor +7 more
TLDR
The conversion of the petrochemical polymer polyethylene terephthalate to a biodegradable plastic polyhydroxyal-kanoate (PHA) is described here and PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium.Abstract:
The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 °C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. freder...read more
Citations
More filters
Journal ArticleDOI
Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates
M. Teresa Cesário,Rodrigo S. Raposo,M. Catarina M.D. de Almeida,Frederik van Keulen,Bruno Sommer Ferreira,M. Manuela R. da Fonseca +5 more
TL;DR: The selected feeding strategy successfully overcame the carbon catabolite repression (CCR) phenomenon observed with sugar mixtures containing hexoses and pentoses, and the P(3HB) volumetric productivities attained are by far the highest ever achieved on agricultural waste hydrolysates.
Journal ArticleDOI
Carbon Sources for Polyhydroxyalkanoates and an Integrated Biorefinery
Guozhan Jiang,David Hill,Marek Kowalczuk,Marek Kowalczuk,Brian Johnston,Grazyna Adamus,Victor U. Irorere,Iza Radecka +7 more
TL;DR: This paper gives an overview of the current carbon sources used for PHA production and the methods used to transform these sources into fermentable forms, and identifies two promising carbon sources for a sustainable production of PHAs.
Journal ArticleDOI
Recent Advances in Bioplastics: Application and Biodegradation.
TL;DR: This review summarises the advances in drug delivery systems, specifically design of nanoparticles based on the biodegradable polymers, and provides an overview of theBiodegradation of these polymers and composites in managed and unmanaged environments.
Book ChapterDOI
Carbon-rich wastes as feedstocks for biodegradable polymer (polyhydroxyalkanoate) production using bacteria.
Jasmina Nikodinovic-Runic,Maciej Guzik,Shane T. Kenny,Ramesh Babu,Alan Werker,Kevin O’ Connor +5 more
TL;DR: This review considers residuals from food, plastic, forest and lignocellulosic, and biodiesel manufacturing (glycerol) to gain perspective of opportunities from literature reporting the production of PHA from carbon-rich residuals as feedstocks.
Journal ArticleDOI
Plastic waste as a novel substrate for industrial biotechnology.
Nick Wierckx,M. Auxiliadora Prieto,Pablo Pomposiello,Víctor de Lorenzo,Kevin E. O’Connor,Lars M. Blank +5 more
TL;DR: It is proposed to use plastic wastes as substrates for the synthesis of added value products, which will empower the recycling industry to a qualitatively new dimension and increase the increase in recycling rates will not be achievable.
References
More filters
Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI
Acetylornithinase of Escherichia coli: partial purification and some properties.
Henry J. Vogel,David M. Bonner +1 more
TL;DR: Compounds Used-N”l-Acetyl-n-ornithine was synthesized as previously described and L-Ornithine monohydrochloride was obtained from the Mann Research Laboratories.
Journal ArticleDOI
Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.
A J Anderson,E A Dawes +1 more
TL;DR: The physiological functions of PHB as a reserve material and in symbiotic nitrogen fixation and its presence in bacterial plasma membranes and putative role in transformability and calcium signaling are also considered.
Journal ArticleDOI
A rapid gas chromatographic method for the determination of poly- β -hydroxybutyric acid in microbial biomass
TL;DR: The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l.
Related Papers (5)
Production, use, and fate of all plastics ever made
Characterization and engineering of a plastic-degrading aromatic polyesterase
Harry P. Austin,Mark D. Allen,Bryon S. Donohoe,Nicholas A. Rorrer,Fiona L. Kearns,Rodrigo L. Silveira,Rodrigo L. Silveira,Benjamin C. Pollard,Graham Dominick,Ramona Duman,Kamel El Omari,Vitaliy Mykhaylyk,Armin Wagner,William E. Michener,Antonella Amore,Munir S. Skaf,Michael F. Crowley,Alan W. Thorne,Christopher W. Johnson,H. Lee Woodcock,John McGeehan,Gregg T. Beckham +21 more