Journal ArticleDOI
Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
Shane T. Kenny,Jasmina Nikodinovic Runic,Walter Kaminsky,Trevor Woods,Ramesh Babu,Chris M. Keely,Werner J. Blau,Kevin E. O’Connor +7 more
TLDR
The conversion of the petrochemical polymer polyethylene terephthalate to a biodegradable plastic polyhydroxyal-kanoate (PHA) is described here and PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium.Abstract:
The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 °C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. freder...read more
Citations
More filters
Journal ArticleDOI
Alcoholysis of poly(ethylene terephthalate) to produce dioctyl terephthalate with sub- and super-critical isooctyl alcohol
TL;DR: In this paper, the alcoholysis of poly(ethylene terephthalate) (PET) to produce Dioctyl Terephthalates (DOTP) was studied using sub- and super-critical isooctyl alcohol (2-EH).
Journal ArticleDOI
Recent developments in bioreactor scale production of bacterial polyhydroxyalkanoates.
Zulfiqar Ali Raza,Muhammad Rizwan Tariq,Muhammad Rizwan Tariq,Muhammad Irfan Majeed,Ibrahim M. Banat +4 more
TL;DR: The present review covers some recent developments in sustainable bioreactor scale production of PHAs and identifies some areas in which future research in this field might be focused.
Journal ArticleDOI
Gene amplification, laboratory evolution, and biosensor screening reveal MucK as a terephthalic acid transporter in Acinetobacter baylyi ADP1.
Isabel Pardo,Ramesh K. Jha,Ryan E. Bermel,Felicia Bratti,Molly Gaddis,Emily A. McIntyre,William E. Michener,Ellen L. Neidle,Taraka Dale,Gregg T. Beckham,Christopher W. Johnson +10 more
TL;DR: This work obtained ADP1-derived strains capable of growing on TPA as the sole carbon source using chromosomal insertion and targeted amplification of the tph catabolic operon from Comamonas sp.
Journal ArticleDOI
High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440
TL;DR: High cell density cultivation is essential for the majority of industrial processes, and this bioprocess represents an excellent basis for the industrial conversion of WCO into biodegradable polymer polyhydroxyalkanoate (PHA).
Journal ArticleDOI
Hydrolysis of waste polyethylene terephthalate catalyzed by easily recyclable terephthalic acid
TL;DR: In this article, the use of TPA, the basic unit of PET, as an acid catalyst to promote the hydrolysis of polyethylene terephthalate (PET) was proposed.
References
More filters
Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI
Acetylornithinase of Escherichia coli: partial purification and some properties.
Henry J. Vogel,David M. Bonner +1 more
TL;DR: Compounds Used-N”l-Acetyl-n-ornithine was synthesized as previously described and L-Ornithine monohydrochloride was obtained from the Mann Research Laboratories.
Journal ArticleDOI
Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.
A J Anderson,E A Dawes +1 more
TL;DR: The physiological functions of PHB as a reserve material and in symbiotic nitrogen fixation and its presence in bacterial plasma membranes and putative role in transformability and calcium signaling are also considered.
Journal ArticleDOI
A rapid gas chromatographic method for the determination of poly- β -hydroxybutyric acid in microbial biomass
TL;DR: The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l.
Related Papers (5)
Production, use, and fate of all plastics ever made
Characterization and engineering of a plastic-degrading aromatic polyesterase
Harry P. Austin,Mark D. Allen,Bryon S. Donohoe,Nicholas A. Rorrer,Fiona L. Kearns,Rodrigo L. Silveira,Rodrigo L. Silveira,Benjamin C. Pollard,Graham Dominick,Ramona Duman,Kamel El Omari,Vitaliy Mykhaylyk,Armin Wagner,William E. Michener,Antonella Amore,Munir S. Skaf,Michael F. Crowley,Alan W. Thorne,Christopher W. Johnson,H. Lee Woodcock,John McGeehan,Gregg T. Beckham +21 more