Journal ArticleDOI
Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
Shane T. Kenny,Jasmina Nikodinovic Runic,Walter Kaminsky,Trevor Woods,Ramesh Babu,Chris M. Keely,Werner J. Blau,Kevin E. O’Connor +7 more
TLDR
The conversion of the petrochemical polymer polyethylene terephthalate to a biodegradable plastic polyhydroxyal-kanoate (PHA) is described here and PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium.Abstract:
The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 °C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. freder...read more
Citations
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Journal ArticleDOI
Biotechnology of Plastic Waste Degradation, Recycling, and Valorization: Current Advances and Future Perspectives
Zi-Hao Qin,Jin Hua Mou,Christopher Yu Huang Chao,Shauhrat S. Chopra,Walid A. Daoud,Shao Yuan Leu,Zhi Ning,Chi Yan Tso,Chak K. Chan,Shixing Tang,Zubeen Hathi,Ariful Haque,Xiang Wang,Carol Sze Ki Lin +13 more
TL;DR: In this article, the collaborative group of Dr. Carol Sze Ki Lin and Dr. Xiang Wang illustrates the biodegradation of plastics and the potential for plastic waste recycling and valorization to address the plastic waste dilemma.
Book ChapterDOI
Host organism: Pseudomonas putida
Ignacio Poblete-Castro,José Manuel Borrero-de Acuña,Pablo I. Nikel,Michael Kohlstedt,Christoph Wittmann +4 more
Journal ArticleDOI
Bioconversion of styrene to poly(hydroxyalkanoate) (PHA) by the new bacterial strain Pseudomonas putida NBUS12.
TL;DR: Results collectively suggested that P. putida NBUS12 is a promising candidate for the biotechnological conversion of styrene into mcl-PHA.
Journal ArticleDOI
News Feature: On the brink of a recycling revolution?
TL;DR: The waste that arrives at MBA Polymers’ big blue factory in Worksop, in England’s industrial heartland, is a dirty, smelly mess that is ground into small 6–8 millimeter particles.
Journal ArticleDOI
Medium‐chain‐length polyhydroxyalkanoate production by newly isolated Pseudomonas sp. TN301 from a wide range of polyaromatic and monoaromatic hydrocarbons
Tanja Narancic,Shane T. Kenny,Lidija Djokic,Branka Vasiljevic,Kevin E. O’Connor,Jasmina Nikodinovic-Runic +5 more
TL;DR: The aim of this study was to convert numerous polyaromatic and monoaromatic hydrocarbons into biodegradable polymer medium‐chain‐length polyhydroxyalkanoate (mcl‐PHA).
References
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Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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Acetylornithinase of Escherichia coli: partial purification and some properties.
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TL;DR: Compounds Used-N”l-Acetyl-n-ornithine was synthesized as previously described and L-Ornithine monohydrochloride was obtained from the Mann Research Laboratories.
Journal ArticleDOI
Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.
A J Anderson,E A Dawes +1 more
TL;DR: The physiological functions of PHB as a reserve material and in symbiotic nitrogen fixation and its presence in bacterial plasma membranes and putative role in transformability and calcium signaling are also considered.
Journal ArticleDOI
A rapid gas chromatographic method for the determination of poly- β -hydroxybutyric acid in microbial biomass
TL;DR: The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l.
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