Journal ArticleDOI
Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
Shane T. Kenny,Jasmina Nikodinovic Runic,Walter Kaminsky,Trevor Woods,Ramesh Babu,Chris M. Keely,Werner J. Blau,Kevin E. O’Connor +7 more
TLDR
The conversion of the petrochemical polymer polyethylene terephthalate to a biodegradable plastic polyhydroxyal-kanoate (PHA) is described here and PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium.Abstract:
The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 °C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. freder...read more
Citations
More filters
Journal ArticleDOI
Pseudomonas as Versatile Aromatics Cell Factory.
TL;DR: Pseudomonads are intriguing hosts for biocatalysis, as they display unique characteristics beneficial for the production of aromatics, including a distinct tolerance and versatile metabolism.
Journal ArticleDOI
Renewable and flexible UV-blocking film from poly(butylene succinate) and lignin
TL;DR: In this paper, a reactive interfacial compatibilization was developed in order to improve the miscibility of lignin with poly(butylene succinate) (PBS) and Lignin.
Journal ArticleDOI
Inspired by nature: Microbial production, degradation and valorization of biodegradable bioplastics for life-cycle-engineered products.
Octavio García-Depraect,Sergio Bordel,Raquel Lebrero,Fernando Santos-Beneit,Rosa Aragão Börner,Tim Börner,Raúl Muñoz +6 more
TL;DR: In this article, a comprehensive overview of microbial-based processes involved in the complete lifecycle of bioplastics is presented, together with the challenges and future perspectives for the development of life-cycle-engineered biobased and biodegradable plastic products.
Journal ArticleDOI
Closed-Loop Polymer Upcycling by Installing Property-Enhancing Comonomer Sequences and Recyclability
TL;DR: The concept of upcycling postconsumer plastics into higher-value products is attractive, but the challenges remain to develop a cost-effective upcycling scheme, discover property-enhancing structur... as mentioned in this paper.
Journal ArticleDOI
The microbial cell factory.
TL;DR: Three of the current applications in which microbes have a significant role to play are highlighted: the discovery of new antibiotics, manufacture of biofuels and bioplastics, and production of fine chemicals via biotransformation.
References
More filters
Journal ArticleDOI
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
Journal ArticleDOI
Acetylornithinase of Escherichia coli: partial purification and some properties.
Henry J. Vogel,David M. Bonner +1 more
TL;DR: Compounds Used-N”l-Acetyl-n-ornithine was synthesized as previously described and L-Ornithine monohydrochloride was obtained from the Mann Research Laboratories.
Journal ArticleDOI
Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.
A J Anderson,E A Dawes +1 more
TL;DR: The physiological functions of PHB as a reserve material and in symbiotic nitrogen fixation and its presence in bacterial plasma membranes and putative role in transformability and calcium signaling are also considered.
Journal ArticleDOI
A rapid gas chromatographic method for the determination of poly- β -hydroxybutyric acid in microbial biomass
TL;DR: The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l.
Related Papers (5)
Production, use, and fate of all plastics ever made
Characterization and engineering of a plastic-degrading aromatic polyesterase
Harry P. Austin,Mark D. Allen,Bryon S. Donohoe,Nicholas A. Rorrer,Fiona L. Kearns,Rodrigo L. Silveira,Rodrigo L. Silveira,Benjamin C. Pollard,Graham Dominick,Ramona Duman,Kamel El Omari,Vitaliy Mykhaylyk,Armin Wagner,William E. Michener,Antonella Amore,Munir S. Skaf,Michael F. Crowley,Alan W. Thorne,Christopher W. Johnson,H. Lee Woodcock,John McGeehan,Gregg T. Beckham +21 more