Journal ArticleDOI
Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
Shane T. Kenny,Jasmina Nikodinovic Runic,Walter Kaminsky,Trevor Woods,Ramesh Babu,Chris M. Keely,Werner J. Blau,Kevin E. O’Connor +7 more
TLDR
The conversion of the petrochemical polymer polyethylene terephthalate to a biodegradable plastic polyhydroxyal-kanoate (PHA) is described here and PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium.Abstract:
The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 °C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. freder...read more
Citations
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Journal ArticleDOI
Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production
Constantina Kourmentza,Jersson Plácido,Nikolaos Venetsaneas,Anna Burniol-Figols,Cristiano Varrone,Hariklia N. Gavala,Maria A.M. Reis +6 more
TL;DR: In this paper, challenges and opportunities regarding polyhydroxyalkanoate production are presented and discussed, covering key steps of their overall production process by applying pure and mixed culture biotechnology, from raw bioprocess development to downstream processing.
Journal ArticleDOI
Polyhydroxyalkanoates: Characteristics, production, recent developments and applications
TL;DR: This review outlines production and characteristics of PHAs, developments in their production, and applications in various industries including nanotechnology.
Journal ArticleDOI
Start a research on biopolymer polyhydroxyalkanoate (PHA) : a review
Giin-Yu Amy Tan,Chia-Lung Chen,Ling Li,Liya Ge,Lin Wang,Indah Mutiara Ningtyas Razaad,Yanhong Li,Lei Zhao,Yu Mo,Jing-Yuan Wang +9 more
TL;DR: This review aims to facilitate the start-up of PHA research by providing a summary of commercially available PHA-accumulating microbial cultures, PHA biosynthetic pathways, and methods for PHA detection, extraction and analysis.
Journal ArticleDOI
Advances and approaches for chemical recycling of plastic waste
Timmy Thiounn,Rhett C. Smith +1 more
Journal ArticleDOI
Microbial and Enzymatic Degradation of Synthetic Plastics
TL;DR: The objective of this review is to outline the advances made in the microbial degradation of synthetic plastics and, overview the enzymes involved in biodegradation.
References
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Journal ArticleDOI
Pseudomonas frederiksbergensis sp. nov., isolated from soil at a coal gasification site.
TL;DR: DNA-DNA hybridization data showed that the isolates from a coal gasification site in Frederiksberg belong to a new species, for which the name Pseudomonas frederiksbergensis sp.
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High yield preparation of genomic DNA from Streptomyces.
TL;DR: The work in the authors’ labs was supported by NIH grants CA72455 (E.S.) and CA28097 (J.K.C.), and grant C017924 from the NYS Health Research Science Board.
Journal ArticleDOI
Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni YZW-D.
TL;DR: Analysis of the catabolic pathways by which isophthalate and terephthalate are degraded demonstrated that a cis-dihydrodiol intermediate is involved in both pathways.
Journal ArticleDOI
Polyhydroxyalkanoate accumulating diversity of Pseudomonas species utilising aromatic hydrocarbons
Karen M. Tobin,Kevin E. O’Connor +1 more
TL;DR: A number of Pseudomonas strains accumulated polyhdroxyalkanoate (PHA) from a variety of aromatic hydrocarbons, suggesting a role for both beta-oxidation and fatty acid synthesis in PHA accumulation from phenylacetic acid.
Journal ArticleDOI
Hydrolysis of poly(ethylene terephthalate) in a fluidised bed reactor
TL;DR: The authors showed that 60% to 72% of terephthalic acid (TA) was recovered from virgin PET in a temperature range between 400 and 500°C, while only 22% to 27% of TA remained in oligomers.
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