Journal ArticleDOI
Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
Shane T. Kenny,Jasmina Nikodinovic Runic,Walter Kaminsky,Trevor Woods,Ramesh Babu,Chris M. Keely,Werner J. Blau,Kevin E. O’Connor +7 more
TLDR
The conversion of the petrochemical polymer polyethylene terephthalate to a biodegradable plastic polyhydroxyal-kanoate (PHA) is described here and PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium.Abstract:
The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 °C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. freder...read more
Citations
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Degradación química del poli(etilen tereftalato) chemical degradation of poly(ethylene terephthalate) degradação química do poli(tereftalato de etileno)
TL;DR: In un tiempo de una hora, el producto de-gradado corresponde probablemente altereftalato de potasio as discussed by the authors, i.e., a 99% de poli(e.g.,
Journal ArticleDOI
Enzyme based recycling processes
TL;DR: In this article , the authors demonstrate how enzyme discovery can lead to more powerful tools for plastics recycling and provide some examples, which can be used for re-synthesis or for bioproduction.
Journal ArticleDOI
Conversion of Polyethylenes into Fungal Secondary Metabolites
TL;DR: In this article , conditions for aerobic, catalytic digestion of polyethylenes collected from post-consumer and oceanic waste streams, creating carboxylic diacids that can then be used as a carbon source by the fungus Aspergillus nidulans.
Journal ArticleDOI
Environmental impacts of microplastic and role of plastisphere microbes in the biodegradation and upcycling of microplastic.
TL;DR: In this paper , a review of the distribution, transportation, transformation, and biodegradation of microplastic in the ecosystem is presented, where the formation of plastisphere by the biofilm-forming microbes has been described.
Posted ContentDOI
Towards Synthetic PETtrophy: Engineering <i>Pseudomonas putida</i> for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression
TL;DR: In this article , Pseudomonas putida was engineered to metabolize the PET and PBAT monomer terephthalic acid (TA) through genomic integration of four tphII operon genes from Comamonas sp . E6.
References
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Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.
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TL;DR: The physiological functions of PHB as a reserve material and in symbiotic nitrogen fixation and its presence in bacterial plasma membranes and putative role in transformability and calcium signaling are also considered.
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