Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania.
Marius Surleac,Ilda Czobor Barbu,Simona Paraschiv,Laura Ioana Popa,Irina Gheorghe,Irina Gheorghe,Luminita Marutescu,Luminita Marutescu,Marcela Popa,Ionela Sarbu,Daniela Tălăpan,Mihai Nita,Alina Viorica Iancu,Manuela Arbune,Alina Manole,Serban Nicolescu,Oana Săndulescu,Adrian Streinu-Cercel,Dan Otelea,Mariana Carmen Chifiriuc +19 more
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The genomic characterization of 47 multi-drug resistant, carbapenem resistant and ESBL-producing K. pneumoniae isolates from the influent versus effluent wastewater samples could be used as markers for the efficiency of the WWTPs in eliminating AR bacteria and ARGs.Abstract:
We report on the genomic characterization of 47 multi-drug resistant, carbapenem resistant and ESBL-producing K. pneumoniae isolates from the influent (I) and effluent (E) of three wastewater treatment plants (WWTPs) and from Romanian hospital units which are discharging the wastewater in the sampled WWTPs. The K. pneumoniae whole genome sequences were analyzed for antibiotic resistance genes (ARGs), virulence genes and sequence types (STs) in order to compare their distribution in C, I and E samples. Both clinical and environmental samples harbored prevalent and widely distributed ESBL genes, i.e. blaSHV, blaOXA, blaTEM and blaCTX M. The most prevalent carbapenemase genes were blaNDM-1, blaOXA-48 and blaKPC-2. They were found in all types of isolates, while blaOXA-162, a rare blaOXA-48 variant, was found exclusively in water samples. A higher diversity of carbapenemases genes was seen in wastewater isolates. The aminoglycoside modifying enzymes (AME) genes found in all types of samples were aac(6'), ant(2'')Ia, aph(3'), aaD, aac(3) and aph(6). Quinolone resistance gene qnrS1 and the multi-drug resistance oqxA/B pump gene were found in all samples, while qnrD and qnrB were associated to aquatic isolates. The antiseptics resistance gene qacEdelta1 was found in all samples, while qacE was detected exclusively in the clinical ones. Trimethroprim-sulfamethoxazole (dfrA, sul1 and sul2), tetracyclines (tetA and tetD) and fosfomycin (fosA6, known to be located on a transpozon) resistance genes were found in all samples, while for choramphenicol and macrolides some ARGs were detected in all samples (catA1 and catB3 / mphA), while other (catA2, cmIA5 and aac(6')Ib / mphE and msrE) only in wastewater samples. The rifampin resistance genes arr2 and 3 (both carried by class I integrons) were detected only in water samples. The highly prevalent ARGs preferentially associating with aquatic versus clinical samples could ascribe potential markers for the aquatic (blaSHV-145, qacEdelta1, sul1, aadA1, aadA2) and clinical (blaOXA-1, blaSHV-106,blaTEM-150, aac(3)Iia, dfrA14, oqxA10; oqxB17,catB3, tetD) reservoirs of AR. Moreover, some ARGs (oqxA10; blaSHV-145; blaSHV-100, aac(6')Il, aph(3')VI, armA, arr2, cmlA5, blaCMY-4, mphE, msrE, oqxB13, blaOXA-10) showing decreased prevalence in influent versus effluent wastewater samples could be used as markers for the efficiency of the WWTPs in eliminating AR bacteria and ARGs. The highest number of virulence genes (75) was recorded for the I samples, while for E and C samples it was reduced to half. The most prevalent belong to three functional groups: adherence (fim genes), iron acquisition (ent, fep, fyu, irp and ybt genes) and the secretion system (omp genes). However, none of the genes associated with hypervirulent K. pneumoniae have been found. A total of 14 STs were identified. The most prevalent clones were ST101, ST219 in clinical samples and ST258, ST395 in aquatic isolates. These STs were also the most frequently associated with integrons. ST45 and ST485 were exclusively associated with I samples, ST11, ST35, ST364 with E and ST1564 with C samples. The less frequent ST17 and ST307 aquatic isolates harbored blaOXA-162, which was co-expressed in our strains with blaCTX-M-15 and blaOXA-1.read more
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The Characteristic of Virulence, Biofilm and Antibiotic Resistance of Klebsiella pneumoniae
TL;DR: This paper systematically summarized the virulence, biofilm, and antibiotic tolerance mechanisms of K. pneumoniae, and explored the application of whole genome sequencing and global proteomics, which will provide new clues for clinical treatment of the pathogen.
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Carbapenemase-producing Gram-negative bacteria in aquatic environments: a review.
TL;DR: In this paper, the authors present current knowledge regarding the spread of carbapenemase-producing isolates in different aquatic environments, which may help the implementation of control and prevention strategies against the spreading of such dangerous resistant agents in the environment.
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Multidrug Resistant Klebsiella pneumoniae ST101 Clone Survival Chain From Inpatients to Hospital Effluent After Chlorine Treatment
Laura Ioana Popa,Irina Gheorghe,Ilda Czobor Barbu,Marius Surleac,Simona Paraschiv,Luminiţa Măruţescu,Marcela Popa,Graţiela Grădişteanu Pîrcălăbioru,Daniela Tălăpan,Mihai Niţă,Anca Streinu-Cercel,Adrian Streinu-Cercel,Dan Oţelea,Mariana Carmen Chifiriuc +13 more
TL;DR: In this paper, the authors describe the transmission of a multi-drug resistant Klebsiella pneumoniae ST101 clone from hospital to wastewater and its persistence after chlorine treatment, raising the risk of surface water contamination and further dissemination to different components of the trophic chain.
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Antibiotics and antibiotic resistant bacteria/genes in urban wastewater: A comparison of their fate in conventional treatment systems and constructed wetlands.
TL;DR: In this paper , the use of constructed wetlands (CWs) has the potential to provide a low-cost solution for wastewater treatment, with respect to removal of nutrients, pathogens, ARB/ARGs either as a standalone treatment process or when integrated with conventional treatment systems.
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Multidrug-Resistant Klebsiella pneumoniae Causing Severe Infections in the Neuro-ICU
Nadezhda K. Fursova,Evgenii I. Astashkin,O.N. Ershova,Irina A. Aleksandrova,Ivan A. Savin,Tatiana S. Novikova,Galina N. Fedyukina,Angelina A. Kislichkina,Mikhail V. Fursov,Ekaterina S Kuzina,Sergei Fedorovich Biketov,I.A. Dyatlov +11 more
TL;DR: In this article, the identification of genetic lineages and virulence genes in Klebsiella pneumoniae isolates associated with severe infections in the neuro-ICU was determined using the Vitek-2 instrument.
References
More filters
Journal ArticleDOI
Basic Local Alignment Search Tool
TL;DR: A new approach to rapid sequence comparison, basic local alignment search tool (BLAST), directly approximates alignments that optimize a measure of local similarity, the maximal segment pair (MSP) score.
Journal ArticleDOI
Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution
Michael R. Gillings,William H. Gaze,Amy Pruden,Kornelia Smalla,James M. Tiedje,Yong-Guan Zhu +5 more
TL;DR: It is suggested that the relative abundance of the clinical class 1 integron-integrase gene, intI1, is a good proxy for pollution because it is linked to genes conferring resistance to antibiotics, disinfectants and heavy metals.
Journal ArticleDOI
Epidemiology of Ciprofloxacin Resistance and Its Relationship to Extended-Spectrum β-Lactamase Production in Klebsiella pneumoniae Isolates Causing Bacteremia
David L. Paterson,Lutfiye Mulazimoglu,Jose Maria Casellas,Wen Chien Ko,Herman Goossens,A. von Gottberg,Sunita Mohapatra,Gordon M. Trenholme,Keith P. Klugman,Joseph G. McCormack,Victor L. Yu +10 more
TL;DR: A prospective study of Klebsiella pneumoniae bacteremia revealed a close relationship between ESBL production and ciprofloxacin resistance, which is particularly worrisome because the first reported instance of plasmid-mediated ciprafloxACin resistance has been in an isolate of K. pneumoniae also possessing an ESBL.
Journal ArticleDOI
Epidemic of carbapenem-resistant Klebsiella pneumoniae in Europe is driven by nosocomial spread
Sophia David,Sandra Reuter,Simon R. Harris,Corinna Glasner,Theresa Feltwell,Silvia Argimón,Khalil Abudahab,Richard Goater,Tommaso Giani,Giulia Errico,Marianne Aspbury,Sara Sjunnebo,Edward J. Feil,Gian Maria Rossolini,David M. Aanensen,Hajo Grundmann,Hajo Grundmann +16 more
TL;DR: Combined analysis of the genetic and geographic distances between isolates with different β-lactam resistance determinants suggests that the propensity of K. pneumoniae to spread in hospital environments correlates with the degree of resistance and that carbapenemase-positive isolates have the highest transmissibility.