Zinc inhibits lethal inflammatory shock by preventing microbe-induced interferon signature in intestinal epithelium.
Jolien Souffriau,Steven Timmermans,Tineke Vanderhaeghen,Charlotte Wallaeys,Kelly Van Looveren,Lindsy Aelbrecht,Sylviane Dewaele,Jolien Vandewalle,Evy Goossens,Serge Verbanck,Filip Boyen,Melanie Eggermont,Lindsey De Commer,Riet De Rycke,Michiel De Bruyne,Raul Y. Tito,Marlies Ballegeer,Sofie Vandevyver,Tiago R. Velho,Luis F. Moita,Tino Hochepied,Karolien De Bosscher,Jeroen Raes,Filip Van Immerseel,Rudi Beyaert,Claude Libert +25 more
TLDR
Zinc modulates the gut microbiota, causing less induction of ISRE/IRF genes in crypt cells, less TNF‐induced necroptosis in Paneth cells, and less fatal evasion of gut bacteria into the system.Abstract:
The cytokine TNF drives inflammatory diseases, e.g., Crohn's disease. In a mouse model of TNF-induced systemic inflammatory response syndrome (SIRS), severe impact on intestinal epithelial cells (IECs) is observed. Zinc confers complete protection in this model. We found that zinc no longer protects in animals which lack glucocorticoids (GCs), or express mutant versions of their receptor GR in IECs, nor in mice which lack gut microbiota. RNA-seq studies in IECs showed that zinc caused reduction in expression of constitutive (STAT1-induced) interferon-stimulated response (ISRE) genes and interferon regulatory factor (IRF) genes. Since some of these genes are involved in TNF-induced cell death in intestinal crypt Paneth cells, and since zinc has direct effects on the composition of the gut microbiota (such as several Staphylococcus species) and on TNF-induced Paneth cell death, we postulate a new zinc-related anti-inflammatory mechanism. Zinc modulates the gut microbiota, causing less induction of ISRE/IRF genes in crypt cells, less TNF-induced necroptosis in Paneth cells, and less fatal evasion of gut bacteria into the system.read more
Citations
More filters
Journal ArticleDOI
Gut microbiota‐derived nicotinamide mononucleotide alleviates acute pancreatitis by activating pancreatic SIRT3 signalling
Li-Wei Liu,Yuxiao Xie,Guanqun Li,Tao Zhang,Y. Sui,Zhongjie Zhao,Yang Zhang,Xinglong Geng,Dongbo Xue,Hua Chen,Yongwei Wang,T. Lu,Li-Ren Shang,Zhibo Li,Le Li,Bei Sun +15 more
TL;DR: In this article , the effect of faecal microbiota transplantation on acute pancreatitis (AP) was investigated and the mechanisms associated with pancreatic damage and inflammation were further clarified.
Journal ArticleDOI
Differential Effects of Transition Metals on Growth and Metal Uptake for Two Distinct Lactobacillus Species
Uyen T.D. Huynh,Muxin Qiao,Jonathan King,Brittany Trinh,Juventino Valdez,Mahmoodul Haq,Melissa L. Zastrow +6 more
TL;DR: Investigation of metal uptake by quantifying total cellular metal contents and compared how transition metals affect the growth of two distinct Lactobacillus species highlights how distinct species can respond differently to varied metal availability and provide a foundation for future molecular and mechanistic studies.
Journal ArticleDOI
Metal ions and nanometallic materials in antitumor immunity: Function, application, and perspective
TL;DR: In this article , the authors focus on the physiopathology of metal ions in tumorigenesis and discuss several breakthroughs regarding the use of nanometallic materials in antitumor immunotherapeutics.
Journal ArticleDOI
Paneth cells as the cornerstones of intestinal and organismal health: a primer
TL;DR: Paneth cells are versatile secretory cells located in the crypts of Lieberkühn of the small intestine as discussed by the authors , and they function as the cornerstones of intestinal health by preserving homeostasis.
Journal ArticleDOI
Metallobiology of Lactobacillaceae in the gut microbiome.
TL;DR: In this article , a review provides a current overview of these mechanisms and covers how iron, zinc and manganese impact Lactobacillaceae in the gut microbiota with an emphasis on their biochemical roles, requirements, and homeostatic mechanisms in several species.
References
More filters
Journal ArticleDOI
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
TL;DR: This work presents DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates, which enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression.
Posted ContentDOI
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
TL;DR: This work presents DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates, which enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression.
Journal ArticleDOI
featureCounts: an efficient general-purpose program for assigning sequence reads to genomic features
TL;DR: FeatureCounts as discussed by the authors is a read summarization program suitable for counting reads generated from either RNA or genomic DNA sequencing experiments, which implements highly efficient chromosome hashing and feature blocking techniques.
Journal ArticleDOI
HISAT: a fast spliced aligner with low memory requirements
TL;DR: Tests showed that HISAT is the fastest system currently available, with equal or better accuracy than any other method, and requires only 4.3 gigabytes of memory.
Journal ArticleDOI
Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
Sven Heinz,Christopher Benner,Nathanael J. Spann,Eric Bertolino,Yin C. Lin,Peter Laslo,Jason X. Cheng,Cornelis Murre,Harinder Singh,Harinder Singh,Christopher K. Glass +10 more
TL;DR: It is demonstrated in macrophages and B cells that collaborative interactions of the common factor PU.1 with small sets of macrophage- or B cell lineage-determining transcription factors establish cell-specific binding sites that are associated with the majority of promoter-distal H3K4me1-marked genomic regions.
Related Papers (5)
Critical Role for a Subset of Intestinal Macrophages in Shaping Gut Microbiota in Adult Zebrafish.
Microbiota maintain colonic homeostasis by activating TLR2/MyD88/PI3K signaling in IL-10–producing regulatory B cells
Yoshiyuki Mishima,Yoshiyuki Mishima,Akihiko Oka,Akihiko Oka,Bo Liu,Jeremy Herzog,Chang Soo Eun,Chang Soo Eun,Ting-Jia Fan,Emily C. Bulik-Sullivan,Ian M. Carroll,Jonathan J. Hansen,Liang Chen,Justin E. Wilson,Nancy C. Fisher,Jenny P.-Y. Ting,Tomonori Nochi,Tomonori Nochi,Angela Wahl,J. Victor Garcia,Christopher L. Karp,R. Balfour Sartor +21 more